Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1111/jam.15443 |
Licence  |
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| Title (Primary) | Structure and functional capacity of a benzene‐mineralizing, nitrate‐reducing microbial community |
| Author | Eziuzor, S.C.; Borim Corrêa, F.; Peng, S.; Schultz, J.; Kleinsteuber, S.
; Nunes da Rocha, U.; Adrian, L.; Vogt, C. |
| Source Titel | Journal of Applied Microbiology |
| Year | 2022 |
| Department | ISOBIO; UMB; UBT |
| Volume | 132 |
| Issue | 4 |
| Page From | 2795 |
| Page To | 2811 |
| Language | englisch |
| Topic | T7 Bioeconomy |
| Supplements | https://sfamjournals.onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1111%2Fjam.15443&file=jam15443-sup-0001-Supinfo.docx https://sfamjournals.onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1111%2Fjam.15443&file=jam15443-sup-0002-TableS2.docx |
| Keywords | anaerobic benzene degradation; dissimilatory nitrate reduction; Peptococcaceae; putative anaerobic benzene carboxylase; anammox bacteria |
| Abstract |
How benzene is metabolized by microbes under anoxic
conditions is not fully understood. Here, we studied the degradation
pathways in a benzene-mineralizing, nitrate-reducing enrichment culture. Benzene mineralization was dependent on the presence of nitrate and correlated to enrichment of a Peptococcaceae
phylotype only distantly related to known anaerobic benzene degraders
of this family. Its relative abundance decreased after benzene
mineralization had terminated, while other abundant taxa - Ignavibacteriaceae, Rhodanobacteraceae and Brocadiaceae
- slightly increased. Generally, the microbial community remained
diverse despite amendment of benzene as single organic carbon source,
suggesting complex trophic interactions between different functional
groups. A subunit of the putative anaerobic benzene carboxylase (AbcA)
previously detected in Peptococcaceae was identified by
metaproteomic analysis suggesting that benzene was activated by
carboxylation. Detection of proteins involved in anaerobic ammonium
oxidation (anammox) indicates that benzene mineralization was
accompanied by anammox, facilitated by nitrite accumulation and the
presence of ammonium in the growth medium. The results suggest that benzene was activated by carboxylation and further assimilated by a novel Peptococcaceae phylotype. The results confirm the hypothesis that Peptococcaceae are important anaerobic benzene degraders. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=25579 |
| Eziuzor, S.C., Borim Corrêa, F., Peng, S., Schultz, J., Kleinsteuber, S., Nunes da Rocha, U., Adrian, L., Vogt, C. (2022): Structure and functional capacity of a benzene‐mineralizing, nitrate‐reducing microbial community J. Appl. Microbiol. 132 (4), 2795 - 2811 10.1111/jam.15443 |
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