Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/j.scitotenv.2021.152704
Licence creative commons licence
Title (Primary) Transport of marine tracer phage particles in soil
Author You, X.; Kallies, R.; Hild, K.; Hildebrandt, A.; Harms, H.; Chatzinotas, A.; Wick, L.Y.
Source Titel Science of the Total Environment
Year 2022
Department CHS; UMB
Volume 814
Page From art. 152704
Language englisch
Topic T7 Bioeconomy
T5 Future Landscapes
Keywords Viruses; Particle tracer; Soil; Breakthrough; Intactness
Abstract Marine phages have been applied to trace ground- and surface water flows. Yet, information on their transport in soil and related particle intactness is limited. Here we compared the breakthrough of two lytic marine tracer phages (Pseudoalteromonas phages PSA-HM1 and PSA-HS2) with the commonly used Escherichia virus T4 in soil- and sand-filled laboratory percolation columns. All three phages showed high mass recoveries in the effluents and a higher transport velocity than non-reactive tracer Br. Comparison of effluent gene copy numbers (CN) to physically-determined phage particle counts or infectious phage counts showed that PSA-HM1 and PSA-HS2 retained high phage particle intactness (Ip > 81%), in contrast to T4 (Ip < 36%). Our data suggest that marine phages may be applied in soil to mimic the transport of (bio-) colloids or anthropogenic nanoparticles of similar traits. Quantitative PCR (qPCR) thereby allows for highly sensitive quantification and thus for the detection of even highly diluted marine tracer phages in environmental samples.
Persistent UFZ Identifier
You, X., Kallies, R., Hild, K., Hildebrandt, A., Harms, H., Chatzinotas, A., Wick, L.Y. (2022):
Transport of marine tracer phage particles in soil
Sci. Total Environ. 814 , art. 152704 10.1016/j.scitotenv.2021.152704