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Title (Primary) Bolstering fitness via CO2 fixation and organic carbon uptake: mixotrophs in modern groundwater
Author Taubert, M.; Overholt, W.A.; Heinze, B.M.; Matanfack, G.A.; Houhou, R.; Jehmlich, N.; von Bergen, M.; Rösch, P.; Popp, J.; Küsel, K.
Journal ISME Journal
Year 2022
Department iDiv; MOLSYB
Volume 16
Issue 4
Page From 1153
Page To 1162
Language englisch
Topic T9 Healthy Planet
Supplements https://static-content.springer.com/esm/art%3A10.1038%2Fs41396-021-01163-x/MediaObjects/41396_2021_1163_MOESM1_ESM.pdf
https://static-content.springer.com/esm/art%3A10.1038%2Fs41396-021-01163-x/MediaObjects/41396_2021_1163_MOESM2_ESM.xlsx
https://static-content.springer.com/esm/art%3A10.1038%2Fs41396-021-01163-x/MediaObjects/41396_2021_1163_MOESM3_ESM.xlsx
https://static-content.springer.com/esm/art%3A10.1038%2Fs41396-021-01163-x/MediaObjects/41396_2021_1163_MOESM4_ESM.xlsx
Abstract Current understanding of organic carbon inputs into ecosystems lacking photosynthetic primary production is predicated on data and inferences derived almost entirely from metagenomic analyses. The elevated abundances of putative chemolithoautotrophs in groundwaters suggest that dark CO2 fixation is an integral component of subsurface trophic webs. To understand the impact of autotrophically fixed carbon, the flux of CO2-derived carbon through various populations of subsurface microbiota must first be resolved, both quantitatively and temporally. Here we implement novel Stable Isotope Cluster Analysis to render a time-resolved and quantitative evaluation of 13CO2-derived carbon flow through a groundwater community in microcosms stimulated with reduced sulfur compounds. We demonstrate that mixotrophs, not strict autotrophs, were the most abundant active organisms in groundwater microcosms. Species of Hydrogenophaga, Polaromonas, Dechloromonas, and other metabolically versatile mixotrophs drove the production and remineralization of organic carbon. Their activity facilitated the replacement of 43% and 80% of total microbial carbon stores in the groundwater microcosms with 13C in just 21 and 70 days, respectively. The mixotrophs employed different strategies for satisfying their carbon requirements by balancing CO2 fixation and uptake of available organic compounds. These different strategies might provide fitness under nutrient-limited conditions, explaining the great abundances of mixotrophs in other oligotrophic habitats, such as the upper ocean and boreal lakes.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=25487
Taubert, M., Overholt, W.A., Heinze, B.M., Matanfack, G.A., Houhou, R., Jehmlich, N., von Bergen, M., Rösch, P., Popp, J., Küsel, K. (2022):
Bolstering fitness via CO2 fixation and organic carbon uptake: mixotrophs in modern groundwater
ISME J. 16 (4), 1153 - 1162