Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/j.envint.2021.106985
Licence creative commons licence
Title (Primary) MAIT cell activation is reduced by direct and microbiota-mediated exposure to bisphenols
Author Krause, J.L.; Engelmann, B.; Nunes da Rocha, U.; Pierzchalski, A.; Chang, H.D.; Zenclussen, A.C.; von Bergen, M.; Rolle-Kampczyk, U.; Herberth, G. ORCID logo
Source Titel Environment International
Year 2022
Department UMB; IMMU; MOLSYB
Volume 158
Page From art. 106985
Language englisch
Topic T9 Healthy Planet
T7 Bioeconomy
Keywords Bisphenols; Human intestinal microbiota; In vitro model; Batch culture; MAIT cells; Immunomodulation
Abstract Oral uptake is the primary route of human bisphenol exposure, resulting in an exposure of the intestinal microbiota and intestine-associated immune cells. Therefore, we compared the impact of bisphenol A (BPA), bisphenol F (BPF) and bisphenol S (BPS) on (i) intestinal microbiota, (ii) microbiota-mediated immunomodulatory effects and (iii) direct effects on mucosal-associated invariant T (MAIT) cells in vitro. We acutely exposed human fecal microbiota, Bacteroides thetaiotaomicron and Escherichia coli to BPA and its analogues BPF and BPS referring to the European tolerable daily intake (TDI), i.e. 2.3 µg/mL, 28.3 µg/mL and 354.0 µg/mL. Growth and viability of E. coli was most susceptible to BPF, whereas B. thetaiotaomicron and fecal microbiota were affected by BPA > BPF > BPS. At 354.0 µg/mL bisphenols altered microbial diversity in compound-specific manner and modulated microbial metabolism, with BPA already acting on metabolism at 28.3 µg/mL. Microbiota-mediated effects on MAIT cells were observed for the individual bacteria at 354.0 µg/mL only. However, BPA and BPF directly modulated MAIT cell responses at low concentrations, whereby bisphenols at concentrations equivalent for the current TDI had no modulatory effects for microbiota or for MAIT cells. Our findings indicate that acute bisphenol exposure may alter microbial metabolism and impact directly on immune cells.
Persistent UFZ Identifier
Krause, J.L., Engelmann, B., Nunes da Rocha, U., Pierzchalski, A., Chang, H.D., Zenclussen, A.C., von Bergen, M., Rolle-Kampczyk, U., Herberth, G. (2022):
MAIT cell activation is reduced by direct and microbiota-mediated exposure to bisphenols
Environ. Int. 158 , art. 106985 10.1016/j.envint.2021.106985