Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1038/s41396-023-01371-7 |
Licence  |
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| Title (Primary) | Mycelial nutrient transfer promotes bacterial co-metabolic organochlorine pesticide degradation in nutrient-deprived environments |
| Author | Khan, N.; Muge, E.; Mulaa, F.J.; Wamalwa, B.; von Bergen, M.; Jehmlich, N.
; Wick, L.Y. |
| Source Titel | ISME Journal |
| Year | 2023 |
| Department | UMB; MOLSYB |
| Volume | 17 |
| Issue | 4 |
| Page From | 570 |
| Page To | 578 |
| Language | englisch |
| Topic | T7 Bioeconomy T9 Healthy Planet |
| Supplements | https://static-content.springer.com/esm/art%3A10.1038%2Fs41396-023-01371-7/MediaObjects/41396_2023_1371_MOESM1_ESM.docx |
| Abstract | Biotransformation of soil organochlorine pesticides (OCP) is often impeded by a lack of nutrients relevant for bacterial growth and/or co-metabolic OCP biotransformation. By providing space-filling mycelia, fungi promote contaminant biodegradation by facilitating bacterial dispersal and the mobilization and release of nutrients in the mycosphere. We here tested whether mycelial nutrient transfer from nutrient-rich to nutrient-deprived areas facilitates bacterial OCP degradation in a nutrient-deficient habitat. The legacy pesticide hexachlorocyclohexane (HCH), a non-HCH-degrading fungus (Fusarium equiseti K3), and a co-metabolically HCH-degrading bacterium (Sphingobium sp. S8) isolated from the same HCH-contaminated soil were used in spatially structured model ecosystems. Using 13C-labeled fungal biomass and protein-based stable isotope probing (protein-SIP), we traced the incorporation of 13C fungal metabolites into bacterial proteins while simultaneously determining the biotransformation of the HCH isomers. The relative isotope abundance (RIA, 7.1–14.2%), labeling ratio (LR, 0.13–0.35), and the shape of isotopic mass distribution profiles of bacterial peptides indicated the transfer of 13C-labeled fungal metabolites into bacterial proteins. Distinct 13C incorporation into the haloalkane dehalogenase (linB) and 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase (LinC), as key enzymes in metabolic HCH degradation, underpin the role of mycelial nutrient transport and fungal-bacterial interactions for co-metabolic bacterial HCH degradation in heterogeneous habitats. Nutrient uptake from mycelia increased HCH removal by twofold as compared to bacterial monocultures. Fungal-bacterial interactions hence may play an important role in the co-metabolic biotransformation of OCP or recalcitrant micropollutants (MPs). |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=25372 |
| Khan, N., Muge, E., Mulaa, F.J., Wamalwa, B., von Bergen, M., Jehmlich, N., Wick, L.Y. (2023): Mycelial nutrient transfer promotes bacterial co-metabolic organochlorine pesticide degradation in nutrient-deprived environments ISME J. 17 (4), 570 - 578 10.1038/s41396-023-01371-7 |
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