Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1111/j.1574-6968.2006.00361.x
Title (Primary) Bacterial extracellular DNA forming a defined network-like structure
Author Böckelmann, U.; Janke, A.; Kuhn, R.; Neu, T.R.; Wecke, J.; Lawrence, J.R.; Szewzyk, U.
Journal FEMS Microbiology Letters
Year 2006
Department FLOEK
Volume 262
Issue 1
Page From 31
Page To 38
Language englisch
Keywords extracellular DNA (eDNA); filamentous network; aquatic bacteria; nanotechnology; nanostructures
Abstract It is generally assumed that nucleic acids are localized inside of living cells and that their primary function is the storage of information. In contrast, extracellular DNA is mainly considered as a remnant of lysed cells. Here, we report the formation of extracellular bacterial DNA as a spatial structure. An aquatic bacterium, strain F8, was isolated, which produced a stable filamentous network of extracellular DNA. Different staining and enzymatic techniques confirmed that it was DNA. We were able to amplify the 16S rRNA gene from the extracellular DNA. Restriction endonuclease cleavage and randomly amplified polymorphic DNA analysis of extracellular and genomic DNAs revealed major similarities, but also some differences in both sequences. Our data demonstrate a new function and relevance for extracellular DNA
Persistent UFZ Identifier
Böckelmann, U., Janke, A., Kuhn, R., Neu, T.R., Wecke, J., Lawrence, J.R., Szewzyk, U. (2006):
Bacterial extracellular DNA forming a defined network-like structure
FEMS Microbiol. Lett. 262 (1), 31 - 38