Publication Details

Category Text Publication
Reference Category Journals
DOI 10.15252/embj.2021108542
Licence creative commons licence
Title (Primary) A dual-function RNA balances carbon uptake and central metabolism in Vibrio cholerae
Author Venkat, K.; Hoyos, M.; Haycocks, J.R.J.; Cassidy, L.; Engelmann, B.; Rolle-Kampczyk, U.; von Bergen, M.; Tholey, A.; Grainger, D.C.; Papenfort, K.
Source Titel EMBO Journal
Year 2021
Department MOLSYB
Volume 40
Issue 24
Page From e108542
Language englisch
Topic T9 Healthy Planet
Data and Software links
Keywords citrate synthase; dual-function RNA; Hfq; small protein; Vibrio cholerae
Abstract Bacterial small RNAs (sRNAs) are well known to modulate gene expression by base pairing with trans-encoded transcripts and are typically non-coding. However, several sRNAs have been reported to also contain an open reading frame and thus are considered dual-function RNAs. In this study, we discovered a dual-function RNA from Vibrio cholerae, called VcdRP, harboring a 29 amino acid small protein (VcdP), as well as a base-pairing sequence. Using a forward genetic screen, we identified VcdRP as a repressor of cholera toxin production and link this phenotype to the inhibition of carbon transport by the base-pairing segment of the regulator. By contrast, we demonstrate that the VcdP small protein acts downstream of carbon transport by binding to citrate synthase (GltA), the first enzyme of the citric acid cycle. Interaction of VcdP with GltA results in increased enzyme activity and together VcdR and VcdP reroute carbon metabolism. We further show that transcription of vcdRP is repressed by CRP allowing us to provide a model in which VcdRP employs two different molecular mechanisms to synchronize central metabolism in V. cholerae.
Persistent UFZ Identifier
Venkat, K., Hoyos, M., Haycocks, J.R.J., Cassidy, L., Engelmann, B., Rolle-Kampczyk, U., von Bergen, M., Tholey, A., Grainger, D.C., Papenfort, K. (2021):
A dual-function RNA balances carbon uptake and central metabolism in Vibrio cholerae
Embo J. 40 (24), e108542 10.15252/embj.2021108542