Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.3390/biom11091391 |
Licence  |
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| Title (Primary) | First dye-decolorizing peroxidase from an ascomycetous fungus secreted by Xylaria grammica |
| Author | Kimani, V.; Ullrich, R.; Büttner, E.; Herzog, R.; Kellner, H.; Jehmlich, N.
; Hofrichter, M.; Liers, C. |
| Source Titel | Biomolecules |
| Year | 2021 |
| Department | MOLSYB |
| Volume | 11 |
| Issue | 9 |
| Page From | art. 1391 |
| Language | englisch |
| Topic | T9 Healthy Planet |
| Supplements | https://www.mdpi.com/2218-273X/11/9/1391/s1 |
| Keywords | dye-decolorizing peroxidase; Xylaria grammica; ascomycete; Mn2+ oxidation; Mn2+ binding site |
| Abstract | Background: Fungal DyP-type peroxidases have so far been described exclusively for basidiomycetes. Moreover, peroxidases from ascomycetes that oxidize Mn2+ ions are yet not known. Methods: We describe here the physicochemical, biocatalytic, and molecular characterization of a DyP-type peroxidase (DyP, EC 1.11.1.19) from an ascomycetous fungus. Results: The enzyme oxidizes classic peroxidase substrates such as 2,6-DMP but also veratryl alcohol and notably Mn2+ to Mn3+ ions, suggesting a physiological function of this DyP in lignin modification. The KM value (49 µM) indicates that Mn2+ ions bind with high affinity to the XgrDyP protein but their subsequent oxidation into reactive Mn3+ proceeds with moderate efficiency compared to MnPs and VPs. Mn2+ oxidation was most effective at an acidic pH (between 4.0 and 5.0) and a hypothetical surface exposed an Mn2+ binding site comprising three acidic amino acids (two aspartates and one glutamate) could be localized within the hypothetical XgrDyP structure. The oxidation of Mn2+ ions is seemingly supported by four aromatic amino acids that mediate an electron transfer from the surface to the heme center. Conclusions: Our findings shed new light on the possible involvement of DyP-type peroxidases in lignocellulose degradation, especially by fungi that lack prototypical ligninolytic class II peroxidases |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=25217 |
| Kimani, V., Ullrich, R., Büttner, E., Herzog, R., Kellner, H., Jehmlich, N., Hofrichter, M., Liers, C. (2021): First dye-decolorizing peroxidase from an ascomycetous fungus secreted by Xylaria grammica Biomolecules 11 (9), art. 1391 10.3390/biom11091391 |
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