Publication Details

Category Text Publication
Reference Category Journals
DOI 10.3390/biom11091391
Licence creative commons licence
Title (Primary) First dye-decolorizing peroxidase from an ascomycetous fungus secreted by Xylaria grammica
Author Kimani, V.; Ullrich, R.; Büttner, E.; Herzog, R.; Kellner, H.; Jehmlich, N. ORCID logo ; Hofrichter, M.; Liers, C.
Source Titel Biomolecules
Year 2021
Department MOLSYB
Volume 11
Issue 9
Page From art. 1391
Language englisch
Topic T9 Healthy Planet
Keywords dye-decolorizing peroxidase; Xylaria grammica; ascomycete; Mn2+ oxidation; Mn2+ binding site
Abstract Background: Fungal DyP-type peroxidases have so far been described exclusively for basidiomycetes. Moreover, peroxidases from ascomycetes that oxidize Mn2+ ions are yet not known. Methods: We describe here the physicochemical, biocatalytic, and molecular characterization of a DyP-type peroxidase (DyP, EC from an ascomycetous fungus. Results: The enzyme oxidizes classic peroxidase substrates such as 2,6-DMP but also veratryl alcohol and notably Mn2+ to Mn3+ ions, suggesting a physiological function of this DyP in lignin modification. The KM value (49 µM) indicates that Mn2+ ions bind with high affinity to the XgrDyP protein but their subsequent oxidation into reactive Mn3+ proceeds with moderate efficiency compared to MnPs and VPs. Mn2+ oxidation was most effective at an acidic pH (between 4.0 and 5.0) and a hypothetical surface exposed an Mn2+ binding site comprising three acidic amino acids (two aspartates and one glutamate) could be localized within the hypothetical XgrDyP structure. The oxidation of Mn2+ ions is seemingly supported by four aromatic amino acids that mediate an electron transfer from the surface to the heme center. Conclusions: Our findings shed new light on the possible involvement of DyP-type peroxidases in lignocellulose degradation, especially by fungi that lack prototypical ligninolytic class II peroxidases
Persistent UFZ Identifier
Kimani, V., Ullrich, R., Büttner, E., Herzog, R., Kellner, H., Jehmlich, N., Hofrichter, M., Liers, C. (2021):
First dye-decolorizing peroxidase from an ascomycetous fungus secreted by Xylaria grammica
Biomolecules 11 (9), art. 1391 10.3390/biom11091391