Publication Details

Category Text Publication
Reference Category Journals
DOI 10.3390/nu13061866
Licence creative commons licence
Title (Primary) The metabolomic-gut-clinical axis of Mankai plant-derived dietary polyphenols
Author Yaskolka Meir, A.; Tuohy, K.; von Bergen, M.; Krajmalnik-Brown, R.; Heinig, U.; Zelicha, H.; Tsaban, G.; Rinott, E.; Kaplan, A.; Aharoni, A.; Zeibich, L.; Chang, D.; Dirks, B.; Diotallevi, C.; Arapitsas, P.; Vrhovsek, U.; Ceglarek, U.; Haange, S.-B. ORCID logo ; Rolle-Kampczyk, U.; Engelmann, B.; Lapidot, M.; Colt, M.; Sun, Q.; Shai, I.
Source Titel Nutrients
Year 2021
Department MOLSYB
Volume 13
Issue 6
Page From art. 1866
Language englisch
Topic T9 Healthy Planet
Keywords Wolffia globosa; polyphenols; flavonoids; plant-based nutrition; weight loss; mediterranean diet
Abstract Background: Polyphenols are secondary metabolites produced by plants to defend themselves from environmental stressors. We explored the effect of Wolffia globosa ‘Mankai’, a novel cultivated strain of a polyphenol-rich aquatic plant, on the metabolomic-gut clinical axis in vitro, in-vivo and in a clinical trial. Methods: We used mass-spectrometry-based metabolomics methods from three laboratories to detect Mankai phenolic metabolites and examined predicted functional pathways in a Mankai artificial-gut bioreactor. Plasma and urine polyphenols were assessed among the 294 DIRECT-PLUS 18-month trial participants, comparing the effect of a polyphenol-rich green-Mediterranean diet (+1240 mg/polyphenols/day, provided by Mankai, green tea and walnuts) to a walnuts-enriched (+440 mg/polyphenols/day) Mediterranean diet and a healthy controlled diet. Results: Approximately 200 different phenolic compounds were specifically detected in the Mankai plant. The Mankai-supplemented bioreactor artificial gut displayed a significantly higher relative-abundance of 16S-rRNA bacterial gene sequences encoding for enzymes involved in phenolic compound degradation. In humans, several Mankai-related plasma and urine polyphenols were differentially elevated in the green Mediterranean group compared with the other groups (p < 0.05) after six and 18 months of intervention (e.g., urine hydroxy-phenyl-acetic-acid and urolithin-A; plasma Naringenin and 2,5-diOH-benzoic-acid). Specific polyphenols, such as urolithin-A and 4-ethylphenol, were directly involved with clinical weight-related changes. Conclusions: The Mankai new plant is rich in various unique potent polyphenols, potentially affecting the metabolomic-gut-clinical axis.
Persistent UFZ Identifier
Yaskolka Meir, A., Tuohy, K., von Bergen, M., Krajmalnik-Brown, R., Heinig, U., Zelicha, H., Tsaban, G., Rinott, E., Kaplan, A., Aharoni, A., Zeibich, L., Chang, D., Dirks, B., Diotallevi, C., Arapitsas, P., Vrhovsek, U., Ceglarek, U., Haange, S.-B., Rolle-Kampczyk, U., Engelmann, B., Lapidot, M., Colt, M., Sun, Q., Shai, I. (2021):
The metabolomic-gut-clinical axis of Mankai plant-derived dietary polyphenols
Nutrients 13 (6), art. 1866 10.3390/nu13061866