Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1002/etc.5153 |
Licence  |
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| Title (Primary) | Removing disturbing matrix constituents from biota extracts from total extraction and silicone-based passive sampling |
| Author | Muz, M.; Rojo-Nieto, E.; Jahnke, A.
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| Source Titel | Environmental Toxicology and Chemistry |
| Year | 2021 |
| Department | OEC; WANA |
| Volume | 40 |
| Issue | 10 |
| Page From | 2693 |
| Page To | 2704 |
| Language | englisch |
| Topic | T9 Healthy Planet |
| Supplements | https://setac.onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fetc.5153&file=etc5153-sup-0001-210607_LipidRemovalPaper_SI_R1.pdf https://setac.onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fetc.5153&file=etc5153-sup-0002-210214_LipidCleanUp_Supp2_R1.xlsx |
| Keywords | Multi-class hydrophobic organic chemicals, biota analysis, GC-HRMS analysis, matrix effects, clean-up, silicone-based passive sampling |
| Abstract | Contaminant analysis in biota extracts can be hampered by matrix interferences caused by, e.g., co-extracted lipids that compromise the quality of the analytical data and require frequent maintenance of the analytical instruments. In this study we aimed to develop and validate a straightforward, robust and reproducible clean-up method with acceptable recoveries for diverse compound classes with a wide range of physico-chemical properties representative of pollutant screening in biota extracts using gas chromatography coupled to high-resolution mass spectrometry (GC-HRMS). We compared Oasis PRiME HLB cartridges, Agilent Captiva EMR-Lipid cartridges and “Freeze-Out” with salmon lipids spiked with 113 target chemicals. The EMR-Lipid cartridges provided extracts with low matrix effects at reproducible recoveries of the multi-class target analytes (93 ± 9% and 95 ± 7% for low and high lipid amounts, respectively). EMR-Lipid cartridges were further tested with spiked pork lipids submitted to total extraction or silicone-based passive sampling. Reproducible recoveries were achieved and matrix residuals were largely removed as demonstrated gravimetrically for both types of extracts. Ion suppression of halogenated compounds was not as efficiently removed by the clean-up of total and silicone-based extracts of pork lipids as for the salmon lipids. However, the samples with clean-up provided better instrument robustness than without clean-up. Hence, EMR-Lipid cartridges were shown to be efficient as a clean-up method in multi-class monitoring of biota samples and open up new possibilities as a suitable clean-up method for silicone extracts in biota passive sampling studies using GC-HRMS. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=24822 |
| Muz, M., Rojo-Nieto, E., Jahnke, A. (2021): Removing disturbing matrix constituents from biota extracts from total extraction and silicone-based passive sampling Environ. Toxicol. Chem. 40 (10), 2693 - 2704 10.1002/etc.5153 |
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