Publication Details

Category Text Publication
Reference Category Journals
DOI 10.18502/ijaai.v21i6.11532
Licence creative commons licence
Title (Primary) Identification of new potential allergens from green-lipped mussel (Perna canaliculus)
Author Kage, P.; Schubert, K.; Treudler, R.; Simon, J.-C.; von Bergen, M.; Tomm, J.
Source Titel Iranian Journal of Allergy Asthma and Immunology
Year 2022
Department MOLSYB
Volume 21
Issue 6
Page From 711
Page To 715
Language englisch
Topic T9 Healthy Planet
Keywords Anaphylaxis; Electrophoresis; IgE; Immunoblotting; Mussel
Abstract The green-lipped mussel (Perna canaliculus) originates from New Zealand. To preserve the health benefits of green-lipped mussel meat, it is freeze-dried to make a long-lasting powder. The powder is used to treat arthritis because of its potential anti-inflammatory properties. The report describes a 54-year-old woman who developed immediate rhinoconjunctival and respiratory symptoms after inhaling green-lipped mussel powder she gave to her dog for arthritis.
A skin prick test with green-lipped mussel powder was performed. Protein extracts from P canaliculus were separated by sodium dodecyl–sulfate polyacrylamide (SDS) gel electrophoresis and probed with serum from patients and serum preincubated with green-lipped mussel extract. Bound immunoglobulin E (IgE) was detected by specific anti-human-IgE antibodies, and IgE-binding proteins were subsequently identified by liquid chromatography and mass spectrometry.
The skin prick test was positive for green-lipped mussel. Specific IgE against green-lipped mussel extract was detected using Western immunoblotting. These potential allergenic proteins were identified by mass spectrometry as actin, tropomyosin, and paramyosin.
All three allergens are reported for the first time for P canaliculus. Actin is a major allergen in Paphia textile, paramyosin in Sarcoptes scarbiei, and tropomyosin in Haliotis discus. For all IgE-binding proteins, the software AllCatPro predicted high allergenicity, supporting our conclusion that these proteins from P canaliculus may also be allergenic. The identification of allergens from P canaliculus provides the opportunity for specific tests to assess the frequency of allergic reactions to P canaliculus.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=24555
Kage, P., Schubert, K., Treudler, R., Simon, J.-C., von Bergen, M., Tomm, J. (2022):
Identification of new potential allergens from green-lipped mussel (Perna canaliculus)
Iran. J. Allergy Asthma Immunol. 21 (6), 711 - 715