Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1007/s00253-021-11249-3
Licence creative commons licence
Title (Primary) Production of nonulosonic acids in the extracellular polymeric substances of “Candidatus Accumulibacter phosphatis”
Author Tomás-Martínez, S.; Kleikamp, H.B.C.; Neu, T.R.; Pabst, M.; Weissbrodt, D.G.; van Loosdrecht, M.C.M.; Lin, Y.
Source Titel Applied Microbiology and Biotechnology
Year 2021
Department FLOEK
Volume 105
Issue 8
Page From 3327
Page To 3338
Language englisch
Topic T5 Future Landscapes
Keywords Nonulosonic acids; Candidatus Accumulibacter phosphatis; Sialic acids; Extracellular polymeric substances; Granular sludge; Biological phosphate removal
Abstract Nonulosonic acids (NulOs) are a family of acidic carbohydrates with a nine-carbon backbone, which include different related structures, such as sialic acids. They have mainly been studied for their relevance in animal cells and pathogenic bacteria. Recently, sialic acids have been discovered as an important compound in the extracellular matrix of virtually all microbial life and in “Candidatus Accumulibacter phosphatis”, a well-studied polyphosphate-accumulating organism, in particular. Here, bioaggregates highly enriched with these bacteria (approx. 95% based on proteomic data) were used to study the production of NulOs in an enrichment of this microorganism. Fluorescence lectin-binding analysis, enzymatic quantification, and mass spectrometry were used to analyze the different NulOs present, showing a wide distribution and variety of these carbohydrates, such as sialic acids and bacterial NulOs, in the bioaggregates. Phylogenetic analysis confirmed the potential of “Ca. Accumulibacter” to produce different types of NulOs. Proteomic analysis showed the ability of “Ca. Accumulibacter” to reutilize and reincorporate these carbohydrates. This investigation points out the importance of diverse NulOs in non-pathogenic bacteria, which are normally overlooked. Sialic acids and other NulOs should be further investigated for their role in the ecology of “Ca. Accumulibacter” in particular, and biofilms in general.
Persistent UFZ Identifier
Tomás-Martínez, S., Kleikamp, H.B.C., Neu, T.R., Pabst, M., Weissbrodt, D.G., van Loosdrecht, M.C.M., Lin, Y. (2021):
Production of nonulosonic acids in the extracellular polymeric substances of “Candidatus Accumulibacter phosphatis”
Appl. Microbiol. Biotechnol. 105 (8), 3327 - 3338 10.1007/s00253-021-11249-3