||Mixing tree species associated with arbuscular or ectotrophic mycorrhizae reveals dual mycorrhization and interactive effects on the fungal partners
||Heklau, H.; Schindler, N.; Buscot, F.; Eisenhauer, N.; Ferlian, O.; Prada Salcedo, L.D.; Bruelheide, H.
||Ecology and Evolution
||T5 Future Landscapes
||arbuscular mycorrhiza; biodiversity‐ecosystem functioning experiment; chernozem; dual mycorrhization; ectomycorrhiza; multi‐trophic interaction; next‐generation sequencing
- Recent studies found that the majority of shrub and
tree species are associated with both arbuscular mycorrhizal (AM) and
ectomycorrhizal (EM) fungi. However, our knowledge on how different
mycorrhizal types interact with each other is still limited. We asked
whether the combination of hosts with a preferred association with
either AM or EM fungi increases the host tree roots’ mycorrhization rate
and affects AM and EM fungal richness and community composition.
- We established a tree diversity experiment, where
five tree species of each of the two mycorrhiza types were planted in
monocultures, two‐species and four‐species mixtures. We applied
morphological assessment to estimate mycorrhization rates and
next‐generation molecular sequencing to quantify mycobiont richness.
- Both the morphological and molecular assessment
revealed dual‐mycorrhizal colonization in 79% and 100% of the samples,
respectively. OTU community composition strongly differed between AM and
EM trees. While host tree species richness did not affect
mycorrhization rates, we observed significant effects of mixing AM‐ and
EM‐associated hosts in AM mycorrhization rate. Glomeromycota richness
was larger in monotypic AM tree combinations than in AM‐EM mixtures,
pointing to a dilution or suppression effect of AM by EM trees. We found
a strong match between morphological quantification of AM
mycorrhization rate and Glomeromycota richness.
Synthesis. We provide evidence that the
combination of hosts differing in their preferred mycorrhiza association
affects the host's fungal community composition, thus revealing
important biotic interactions among trees and their associated fungi.
|Persistent UFZ Identifier
|Heklau, H., Schindler, N., Buscot, F., Eisenhauer, N., Ferlian, O., Prada Salcedo, L.D., Bruelheide, H. (2021):
Mixing tree species associated with arbuscular or ectotrophic mycorrhizae reveals dual mycorrhization and interactive effects on the fungal partners
Ecol. Evol. 11 (10), 5424 - 5440