Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1038/s41598-021-85594-8
Licence creative commons licence
Title (Primary) New thermodynamic activity-based approach allows predicting the feasibility of glycolysis
Author Greinert, T.; Vogel, K.; Maskow, T. ORCID logo ; Held, C.
Source Titel Scientific Reports
Year 2021
Department UMB
Volume 11
Page From art. 6125
Language englisch
Topic T7 Bioeconomy
Supplements https://static-content.springer.com/esm/art%3A10.1038%2Fs41598-021-85594-8/MediaObjects/41598_2021_85594_MOESM1_ESM.pdf
Abstract Thermodynamic feasibility analyses help evaluating the feasibility of metabolic pathways. This is an important information used to develop new biotechnological processes and to understand metabolic processes in cells. However, literature standard data are uncertain for most biochemical reactions yielding wrong statements concerning their feasibility. In this article we present activity-based equilibrium constants for all the ten glycolytic reactions, accompanied by the standard reaction data (standard Gibbs energy of reaction and standard enthalpy of reaction). We further developed a thermodynamic activity-based approach that allows to correctly determine the feasibility of glycolysis under different chosen conditions. The results show for the first time that the feasibility of glycolysis can be explained by thermodynamics only if (1) correct standard data are used and if (2) the conditions in the cell at non-equilibrium states are accounted for in the analyses. The results here will help to determine the feasibility of other metabolisms and to understand metabolic processes in cells in the future.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=24417
Greinert, T., Vogel, K., Maskow, T., Held, C. (2021):
New thermodynamic activity-based approach allows predicting the feasibility of glycolysis
Sci. Rep. 11 , art. 6125 10.1038/s41598-021-85594-8