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Title (Primary) Evaluation of two methods to concentrate SARS-CoV-2 from untreated wastewater
Author Dumke, R.; de la Cruz Barron, M.; Oertel, R.; Helm, B.; Kallies, R.; Berendonk, T.U.; Dalpke, A.;
Journal Pathogens
Year 2021
Department UMB; FLOEK;
Volume 10
Issue 2
Language englisch;
Supplements https://www.mdpi.com/2076-0817/10/2/195/s1
Keywords SARS-CoV-2; wastewater; virus concentration; wastewater-based epidemiology; monitoring; surveillance
Abstract Use of wastewater-based epidemiology as a tool to record and manage the course of SARS-CoV-2 infections in human populations requires information about the efficiency of methods to concentrate the virus from wastewater. In the present study, we spiked untreated wastewater with quantified SARS-CoV-2 positive clinical material and enriched the virus by polyethylene glycol precipitation and ultrafiltration with Vivaspin 10 kDa MWCO columns. SARS-CoV-2 was detected and quantified by reverse transcription quantitative PCR (E- and S-gene) and droplet digital PCR. The concentration of virus with precipitation resulted in mean recoveries between 59.4% and 63.7% whereas rates from 33.0% to 42.6% after ultrafiltration of samples were demonstrated. The results suggest that the use of both methods allows an effective and practicable enrichment of SARS-CoV-2 from raw wastewater.
ID 24266
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=24266
Dumke, R., de la Cruz Barron, M., Oertel, R., Helm, B., Kallies, R., Berendonk, T.U., Dalpke, A. (2021):
Evaluation of two methods to concentrate SARS-CoV-2 from untreated wastewater
Pathogens 10 (2), art. 195