Publication Details

Category Text Publication
Reference Category Journals
DOI 10.3390/microorganisms9010003
Licence creative commons licence
Title (Primary) Comparative proteomics of Marinobacter sp. TT1 reveals corexit impacts on hydrocarbon metabolism, chemotactic motility, and biofilm formation
Author Rughöft, S.; Jehmlich, N. ORCID logo ; Gutierrez, T.; Kleindienst, S.
Source Titel Microorganisms
Year 2021
Department MOLSYB
Volume 9
Issue 1
Page From art. 3
Language englisch
Topic T9 Healthy Planet
Supplements https://www.mdpi.com/2076-2607/9/1/3/s1
Keywords Marinobacter; dispersant; Corexit; WAF; hexadecane; hydrocarbon metabolism; proteomics; chemotactic motility; biofilm formation
Abstract The application of chemical dispersants during marine oil spills can affect the community composition and activity of marine microorganisms. Several studies have indicated that certain marine hydrocarbon-degrading bacteria, such as Marinobacter spp., can be inhibited by chemical dispersants, resulting in lower abundances and/or reduced biodegradation rates. However, a major knowledge gap exists regarding the mechanisms underlying these physiological effects. Here, we performed comparative proteomics of the Deepwater Horizon isolate Marinobacter sp. TT1 grown under different conditions. Strain TT1 received different carbon sources (pyruvate vs. n-hexadecane) with and without added dispersant (Corexit EC9500A). Additional treatments contained crude oil in the form of a water-accommodated fraction (WAF) or chemically-enhanced WAF (CEWAF; with Corexit). For the first time, we identified the proteins associated with alkane metabolism and alginate biosynthesis in strain TT1, report on its potential for aromatic hydrocarbon biodegradation and present a protein-based proposed metabolism of Corexit components as carbon substrates. Our findings revealed that Corexit exposure affects hydrocarbon metabolism, chemotactic motility, biofilm formation, and induces solvent tolerance mechanisms, like efflux pumps, in strain TT1. This study provides novel insights into dispersant impacts on microbial hydrocarbon degraders that should be taken into consideration for future oil spill response actions.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=24241
Rughöft, S., Jehmlich, N., Gutierrez, T., Kleindienst, S. (2021):
Comparative proteomics of Marinobacter sp. TT1 reveals corexit impacts on hydrocarbon metabolism, chemotactic motility, and biofilm formation
Microorganisms 9 (1), art. 3