Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1002/cyto.a.20661
Title (Primary) Prediction of flocculation ability of brewing yeast inoculates by flow cytometry, proteome analysis, and mRNA profiling
Author Heine, F.; Stahl, F.; Sträuber, H.; Wiacek, C.; Benndorf, D.; Repenning, C.; Schmidt, F.; Scheper, T.; von Bergen, M.; Harms, H.; Müller, S.
Source Titel Cytometry Part A
Year 2009
Department UMB; METABOX; PROTEOM
Volume 75A
Issue 2
Page From 140
Page To 147
Language englisch
Keywords flocculation; Lg-Flo1p; Saccharomyces cerevisiae; proteome; microarray; microbial flow cytometry
Abstract The ability of brewing yeast to flocculate is an important feature for brewing of qualitatively good beer. Flocculation involves two main cell wall structures, which are the flocculation proteins (flocculins) and mannans, to which these flocculins bind. Unfortunately, in practice, the flocculation ability may get lost after several repitches. Flow cytometry was employed to analyze glucose and mannose structures of the cell surface by application of fluorescent lectins. Validation of the expression of the flocculin genes Lg-FLO1, FLO1, FLO5, and FLO9 was carried out using microarray techniques. SDS-PAGE, western blot, and ESI-MS/MS analyses served to isolate and determine yeast cell flocculins. Mannose and glucose labeling with fluorescent lectins allowed differentiating powdery and flocculent yeast cells under laboratory conditions. Using microarray techniques and proteomics, the four flocculation genes Lg-FLO1, FLO1, FLO5, FLO9, and the protein Lg-Flo1p were identified as f! actors of major importance for flocculation. The expression of the genes was several times higher in flocculent yeast cells than in powdery ones. Flow cytometry is a fast and simple method to quantify the proportions of powdery and flocculent yeast cells in suspensions under defined cultivation conditions. However, differentiation under industrial conditions will require mRNA and protein expression profiling. (C) 2008 international Society for Advancement of Cytometry
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=242
Heine, F., Stahl, F., Sträuber, H., Wiacek, C., Benndorf, D., Repenning, C., Schmidt, F., Scheper, T., von Bergen, M., Harms, H., Müller, S. (2009):
Prediction of flocculation ability of brewing yeast inoculates by flow cytometry, proteome analysis, and mRNA profiling
Cytom. Part A 75A (2), 140 - 147 10.1002/cyto.a.20661