Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/j.jdent.2020.103555
Title (Primary) Antibacterial effect of a brominated self-etch adhesive on carious dentin - An in vivo study
Author Schmidt, J.; Krohn, S.; Kallies, R.; Schneider, H.; Zeller, K.; Ziebolz, D.; Berg, T.; Haak, R.
Journal Journal of Dentistry
Year 2021
Department UMB
Volume 105
Page From art. 103555
Language englisch
Topic T7 Bioeconomy
Keywords Carious dentin; Selective caries removal; Antibacterial adhesives; Brominated MDP; Bacterial DNA; 16S rRNA gene sequencing; Microbiome


This in vivo study compared the antibacterial effect of a self-etch adhesive with and without the brominated monomer 12-methacryloyloxydodecyl-pyridinium bromide (MDPB) on carious dentin after selective caries removal.


10 patients showing deep primary carious lesions at two posterior teeth without pulpal symptoms were included. At visit I, carious tissue was selectively removed and carious dentin was sampled with a sterile roundbur (Komet No. 18). One cavity was restored with composite (SDR, Ceram X; DENTSPLY DeTrey) using an MDPB-containing self-etch adhesive (Clearfil Protect Bond, Kuraray Noritake; PB). The other restoration served as a control (Clearfil SE Bond II, Kuraray Noritake; SE). At visit II after 8 weeks, carious dentin was sampled again. Bacterial growth in carious dentin was differentiated using microbial cultivation. Bacterial DNA from intact cells and cell-free DNA were quantified using 16S rRNA gene-based real-time PCR and the microbial community composition was analyzed by amplicon deep-sequencing. Wilcoxon test was applied for statistical analysis.


Both treatments showed a decrease of intact bacterial cells in carious dentin at visit II compared to visit I (PB: visit I: 1.1*106, visit II: 1.7*105 (p = 0.03); SE: visit I: 1.1*107, visit II = 2.4*105 (p = 0.002)). No statistically significant reduction of cell-free bacterial DNA was detected (PB: visit I: 6.1*105, visit II: 1.6*105 (p = 0.08); SE: visit I: 5.3*105, visit II: 2.9*105 (p = 0.10)). The decrease of intact cell-derived (p = 0.371) and cell-free DNA (p = 0.455) did not differ significantly between PB and SE. Lactobacillus was most abundant within the microbial community at both visits. Alpha-diversity was not affected by treatment and samples showed high intra- and interindividual diversity.

Conclusion and clinical significance

Both self-etch adhesives have an antibacterial effect due to a decrease of bacterial DNA after selective caries removal. However, the results do not reveal any additional antibacterial effect by MDPB.

The study is registered with the German Clinical Trials Register (DRKS00011532).

Persistent UFZ Identifier
Schmidt, J., Krohn, S., Kallies, R., Schneider, H., Zeller, K., Ziebolz, D., Berg, T., Haak, R. (2021):
Antibacterial effect of a brominated self-etch adhesive on carious dentin - An in vivo study
J. Dent. 105 , art. 103555