Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1080/10256019508036284 |
| Title (Primary) | 15N/14N analysis of amino acids with GC-C-IRMS - Methodical investigations and ecotoxicological applications |
| Author | Hofmann, D.; Jung, K.; Segschneider, H.-J.; Gehre, M.; Schüürmann, G. |
| Journal | Isotopes in Environmental and Health Studies |
| Year | 1995 |
| Department | ISOBIO; OEC; COE; BOFO |
| Volume | 31 |
| Issue | 3-4 |
| Page From | 367 |
| Page To | 375 |
| Language | englisch |
| Keywords | amino acids; derivatization; GC-C-IRMS; natural variations; nitrogen 15; sample preparation |
| Abstract | In order to perform the 15N/14N analysis of amino acids using a gas chromatograph and isotope ratio mass spectrometer linked up via a combustion interface (GC-C-IRMS), the amino acids must be derivatized. Tert-butyldimethylsilylation is examined using various techniques (direct conversion to nitrogen gas, ConFlo-IRMS [1], GC-C-IRMS [2]) and subsequently applied to isotopic characterization of amino acids from wheat protein hydrolyzate obtained from plants exposed to ozone. The method provides a reliable tool for studying ecotoxicological effects on plants at a molecular level in addition to the investigation of the natural variations of different N fractions. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=24090 |
| Hofmann, D., Jung, K., Segschneider, H.-J., Gehre, M., Schüürmann, G. (1995): 15N/14N analysis of amino acids with GC-C-IRMS - Methodical investigations and ecotoxicological applications Isot. Environ. Health Stud. 31 (3-4), 367 - 375 |
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