Publication Details

Category Text Publication
Reference Category Journals
DOI 10.3390/microorganisms8121959
Licence creative commons licence
Title (Primary) Coupling an electroactive Pseudomonas putida KT2440 with bioelectrochemical rhamnolipid production
Author Askitosari, T.D.; Berger, C.; Tiso, T.; Harnisch, F. ORCID logo ; Blank, L.M.; Rosenbaum, M.A.
Source Titel Microorganisms
Year 2020
Department UMB
Volume 8
Issue 12
Page From art. 1959
Language englisch
Keywords Pseudomonas putida; rhamnolipid; bioelectrochemical system; phenazines; redox mediator; microbial electrosynthesis; electrobiotechnology; metabolic engineering
Abstract Sufficient supply of oxygen is a major bottleneck in industrial biotechnological synthesis. One example is the heterologous production of rhamnolipids using Pseudomonas putida KT2440. Typically, the synthesis is accompanied by strong foam formation in the reactor vessel hampering the process. It is caused by the extensive bubbling needed to sustain the high respirative oxygen demand in the presence of the produced surfactants. One way to reduce the oxygen requirement is to enable the cells to use the anode of a bioelectrochemical system (BES) as an alternative sink for their metabolically derived electrons. We here used a P. putida KT2440 strain that interacts with the anode using mediated extracellular electron transfer via intrinsically produced phenazines, to perform heterologous rhamnolipid production under oxygen limitation. The strain P. putida RL-PCA successfully produced 30.4 ± 4.7 mg/L mono-rhamnolipids together with 11.2 ± 0.8 mg/L of phenazine-1-carboxylic acid (PCA) in 500-mL benchtop BES reactors and 30.5 ± 0.5 mg/L rhamnolipids accompanied by 25.7 ± 8.0 mg/L PCA in electrode containing standard 1-L bioreactors. Hence, this study marks a first proof of concept to produce glycolipid surfactants in oxygen-limited BES with an industrially relevant strain
Persistent UFZ Identifier
Askitosari, T.D., Berger, C., Tiso, T., Harnisch, F., Blank, L.M., Rosenbaum, M.A. (2020):
Coupling an electroactive Pseudomonas putida KT2440 with bioelectrochemical rhamnolipid production
Microorganisms 8 (12), art. 1959 10.3390/microorganisms8121959