Publication Details

Category Text Publication
Reference Category Journals
DOI 10.3390/metabo4020453
Licence creative commons licence
Title (Primary) Surveillance of C-allocation in microalgal cells
Author Wagner, H.; Jungandreas, A.; Fanesi, A.; Wilhelm, C.
Source Titel Metabolites
Year 2014
Department CLE
Volume 4
Issue 2
Page From 453
Page To 464
Language englisch
Keywords FTIR spectroscopy; algae; biomass; composition; metabolomics; chemometrics; Chlamydomonas
Abstract When microalgae are exposed to changing environmental conditions, e.g., light-dark cycles or oscillations in nutrient availability (CO2, nitrogen, phosphate or silicate) they respond with metabolic changes in the carbon allocation pattern. Short time regulations in the time range of few seconds to minutes can be mirrored best by mass spectroscopy based metabolomics. However, these snap shots do not reflect the alterations in the carbon flow to the cellular macromolecules like protein, carbohydrate or lipid. In this review it is shown how the combination of FTIR spectroscopy and Chla-in-vivo-fluorescence based electron transport rates can reveal changes in the metabolic flux rates of carbon during a shift of the environmental conditions. The review will demonstrate in which time range FTIR spectroscopy can deliver significant information and how FTIR spectroscopy data can synergistically support metabolome analysis by mass-spectroscopy.
Persistent UFZ Identifier
Wagner, H., Jungandreas, A., Fanesi, A., Wilhelm, C. (2014):
Surveillance of C-allocation in microalgal cells
Metabolites 4 (2), 453 - 464 10.3390/metabo4020453