Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1038/s41467-020-18928-1
Licence creative commons licence
Title (Primary) Rational design of a microbial consortium of mucosal sugar utilizers reduces Clostridiodes difficile colonization
Author Pereira, F.C.; Wasmund, K.; Cobankovic, I.; Jehmlich, N. ORCID logo ; Herbold, C.W.; Lee, K.S.; Sziranyi, B.; Vesely, C.; Decker, T.; Stocker, R.; Warth, B.; von Bergen, M.; Wagner, M.; Berry, D.
Source Titel Nature Communications
Year 2020
Department MOLSYB
Volume 11
Page From art. 5104
Language englisch
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Abstract Many intestinal pathogens, including Clostridioides difficile, use mucus-derived sugars as crucial nutrients in the gut. Commensals that compete with pathogens for such nutrients are therefore ecological gatekeepers in healthy guts, and are attractive candidates for therapeutic interventions. Nevertheless, there is a poor understanding of which commensals use mucin-derived sugars in situ as well as their potential to impede pathogen colonization. Here, we identify mouse gut commensals that utilize mucus-derived monosaccharides within complex communities using single-cell stable isotope probing, Raman-activated cell sorting and mini-metagenomics. Sequencing of cell-sorted fractions reveals members of the underexplored family Muribaculaceae as major mucin monosaccharide foragers, followed by members of Lachnospiraceae, Rikenellaceae, and Bacteroidaceae families. Using this information, we assembled a five-member consortium of sialic acid and N-acetylglucosamine utilizers that impedes C. difficile’s access to these mucosal sugars and impairs pathogen colonization in antibiotic-treated mice. Our findings underscore the value of targeted approaches to identify organisms utilizing key nutrients and to rationally design effective probiotic mixtures.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=23883
Pereira, F.C., Wasmund, K., Cobankovic, I., Jehmlich, N., Herbold, C.W., Lee, K.S., Sziranyi, B., Vesely, C., Decker, T., Stocker, R., Warth, B., von Bergen, M., Wagner, M., Berry, D. (2020):
Rational design of a microbial consortium of mucosal sugar utilizers reduces Clostridiodes difficile colonization
Nat. Commun. 11 , art. 5104 10.1038/s41467-020-18928-1