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Title (Primary) Rational design of a microbial consortium of mucosal sugar utilizers reduces Clostridiodes difficile colonization
Author Pereira, F.C.; Wasmund, K.; Cobankovic, I.; Jehmlich, N.; Herbold, C.W.; Lee, K.S.; Sziranyi, B.; Vesely, C.; Decker, T.; Stocker, R.; Warth, B.; von Bergen, M.; Wagner, M.; Berry, D.;
Journal Nature Communications
Year 2020
Department MOLSYB;
Volume 11
Language englisch;
POF III (all) F11;
Data links https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-020-18928-1/MediaObjects/41467_2020_18928_MOESM7_ESM.xlsx
Supplements https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-020-18928-1/MediaObjects/41467_2020_18928_MOESM1_ESM.pdf
https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-020-18928-1/MediaObjects/41467_2020_18928_MOESM3_ESM.docx
https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-020-18928-1/MediaObjects/41467_2020_18928_MOESM4_ESM.xlsx
https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-020-18928-1/MediaObjects/41467_2020_18928_MOESM5_ESM.xlsx
Abstract Many intestinal pathogens, including Clostridioides difficile, use mucus-derived sugars as crucial nutrients in the gut. Commensals that compete with pathogens for such nutrients are therefore ecological gatekeepers in healthy guts, and are attractive candidates for therapeutic interventions. Nevertheless, there is a poor understanding of which commensals use mucin-derived sugars in situ as well as their potential to impede pathogen colonization. Here, we identify mouse gut commensals that utilize mucus-derived monosaccharides within complex communities using single-cell stable isotope probing, Raman-activated cell sorting and mini-metagenomics. Sequencing of cell-sorted fractions reveals members of the underexplored family Muribaculaceae as major mucin monosaccharide foragers, followed by members of Lachnospiraceae, Rikenellaceae, and Bacteroidaceae families. Using this information, we assembled a five-member consortium of sialic acid and N-acetylglucosamine utilizers that impedes C. difficile’s access to these mucosal sugars and impairs pathogen colonization in antibiotic-treated mice. Our findings underscore the value of targeted approaches to identify organisms utilizing key nutrients and to rationally design effective probiotic mixtures.
ID 23883
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=23883
Pereira, F.C., Wasmund, K., Cobankovic, I., Jehmlich, N., Herbold, C.W., Lee, K.S., Sziranyi, B., Vesely, C., Decker, T., Stocker, R., Warth, B., von Bergen, M., Wagner, M., Berry, D. (2020):
Rational design of a microbial consortium of mucosal sugar utilizers reduces Clostridiodes difficile colonization
Nat. Commun. 11 , art. 5104