Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1186/s13068-020-01825-6 |
Licence  |
|
| Title (Primary) | Evolutionary engineering of E. coli MG1655 for tolerance against isoprenol |
| Author | Babel, H.; Krömer, J.O. |
| Source Titel | Biotechnology for Biofuels |
| Year | 2020 |
| Department | SOMA |
| Volume | 13 |
| Page From | art. 183 |
| Language | englisch |
| Supplements | https://static-content.springer.com/esm/art%3A10.1186%2Fs13068-020-01825-6/MediaObjects/13068_2020_1825_MOESM1_ESM.pdf |
| Keywords | Adaptive laboratory evolution; E. coli; Isoprenol; Butanol; Tolerance; Terpenes |
| Abstract | Background: Isoprenol is the basis for industrial flavor and vitamin synthesis and also a promising biofuel. Biotechnological production of isoprenol with E. coli is currently limited by the high toxicity of the final product. Adaptive laboratory evolution (ALE) is a promising method to address complex biological problems such as toxicity. Results: Here we applied this method successfully to evolve E. coli towards higher tolerance against isoprenol, increasing growth at the half-maximal inhibitory concentration by 47%. Whole-genome re-sequencing of strains isolated from three replicate evolutions at seven time-points identified four major target genes for isoprenol tolerance: fabF, marC, yghB, and rob. We could show that knock-out of marC and expression of mutated Rob H(48) → frameshift increased tolerance against isoprenol and butanol. RNA-sequencing showed that the deletion identified upstream of yghB correlated with a strong overexpression of the gene. The knock-out of yghB demonstrated that it was essential for isoprenol tolerance. The mutated Rob protein and yghB deletion also lead to increased vanillin tolerance. Conclusion: Through ALE, novel targets for strain optimization in isoprenol production and also the production of other fuels, such as butanol, could be obtained. Their effectiveness could be shown through re-engineering. This paves the way for further optimization of E. coli for biofuel production. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=23839 |
| Babel, H., Krömer, J.O. (2020): Evolutionary engineering of E. coli MG1655 for tolerance against isoprenol Biotechnol. Biofuels 13 , art. 183 10.1186/s13068-020-01825-6 |
|