Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1016/j.jhazmat.2020.124301 |
| Document | accepted manuscript |
| Title (Primary) | Characterizing the biotransformation of hexachlorocyclohexanes in wheat using compound-specific stable isotope analysis and enantiomer fraction analysis |
| Author | Liu, X.; Wu, L.; Kümmel, S.
; Richnow, H.H. |
| Source Titel | Journal of Hazardous Materials |
| Year | 2021 |
| Department | ISOBIO |
| Volume | 406 |
| Page From | art. 124301 |
| Language | englisch |
| Topic | T7 Bioeconomy |
| Keywords | Hexachlorocyclohexane isomers; isotope fractionation; enantiomer fraction; plant transformation |
| UFZ wide themes | ProVIS; |
| Abstract | Hexachlorocyclohexane isomers (HCHs) are persistent organic pollutants being responsible for environmental contamination worldwide. In order to characterize transformation of HCHs in different plant compartments during uptake, a hydroponic experimental setup was designed using wheat as the test plant. The extent of transformation was determined by using compound-specific isotope analysis (CSIA) and enantiomer fraction (EF) analysis. In nutrient solutions, no change of carbon (δ13C) and chlorine isotope ratios (δ37Cl) of α-HCH and β-HCH was detected throughout the experiment indicating no transformation there. In wheat leaves, stems and roots, however, transformation of α-HCH due to a C-Cl bond cleavage was indicated by increasing δ13C and δ37Cl compared to the nutrient solution. In addition, 1,3,4,5,6-pentachlorocyclohexene (PCCH) was identified as the major metabolite of α-HCH transformation. For β-HCH, in contrast, no transformation was detected. The evaluation of enantiomer fraction analysis revealed no change of the EF(-) in the nutrient solution or on root surface but a decrease in the wheat compartments, providing an evidence for the preferential biological transformation of (-) α-HCH in wheat. The current study provides the first experimental evidence for biotransformation of α-HCH in wheat using CSIA and EF and provides a concept to evaluate processes during phytoremediation. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=23791 |
| Liu, X., Wu, L., Kümmel, S., Richnow, H.H. (2021): Characterizing the biotransformation of hexachlorocyclohexanes in wheat using compound-specific stable isotope analysis and enantiomer fraction analysis J. Hazard. Mater. 406 , art. 124301 10.1016/j.jhazmat.2020.124301 |
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