|Title (Primary)||Soil texture, sampling depth and root hairs shape the structure of ACC deaminase bacterial community composition in maize rhizosphere|
|Author||Gebauer, L.; Bouffaud, M.-L.; Ganther, M.; Yim, B.; Vetterlein, D.; Smalla, K.; Buscot, F.; Heintz-Buschart, A.; Tarkka, M.|
|Journal||Frontiers in Microbiology|
|Department||BOOEK; iDiv; BOSYS|
|Page From||art. 616828|
|Topic||T5 Future Landscapes|
|Keywords||ethylene; rhizosphere; Soil; root; Plant-Microbe Interactions; PGPR; crop|
|Abstract||Preservation of the phytostimulatory functions of plant growth-promoting
bacteria relies on the adaptation of their community to the rhizosphere
environment. Here, an amplicon sequencing approach was implemented to
specifically target microorganisms with
1-aminocyclopropane-1-carboxylate deaminase activity, carrying the acdS
gene. We stated the hypothesis that the relative phylogenetic
distribution of acdS carrying microorganisms is affected by the presence
or absence of root hairs, soil type and depth. To this end, a
standardized soil column experiment was conducted with maize wild type
and root hair defective rth3 mutant in the substrates loam and sand, and
harvest was implemented from three depths.
Most acdS sequences (99%) were affiliated to Actinobacteria and Proteobacteria, and the strongest influence on the relative abundances of sequences were exerted by the substrate. Variovorax, Acidovorax and Ralstonia sequences dominated in loam, whereas Streptomyces and Agromyces were more abundant in sand. Soil depth caused strong variations in acdS sequence distribution, with differential levels in the relative abundances of acdS sequences affiliated to Tetrasphaera, Amycolatopsis and Streptomyces in loam, but Burkholderia, Paraburkholderia and Variovorax in sand. Maize genotype influenced the distribution of acdS sequences mainly in loam and only in the uppermost depth. Variovorax acdS sequences were more abundant in WT, but Streptomyces, Microbacterium and Modestobacter in rth3 rhizosphere.
Substrate and soil depth were strong and plant genotype a further significant single and interacting drivers of acdS carrying microbial community composition in the rhizosphere of maize. This suggests that maize rhizosphere acdS carrying bacterial community establishes according to the environmental constraints, and that root hairs possess a minor but significant impact on acdS carrying bacterial populations.
|Persistent UFZ Identifier||https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=23752|
|Gebauer, L., Bouffaud, M.-L., Ganther, M., Yim, B., Vetterlein, D., Smalla, K., Buscot, F., Heintz-Buschart, A., Tarkka, M. (2021):
Soil texture, sampling depth and root hairs shape the structure of ACC deaminase bacterial community composition in maize rhizosphere
Front. Microbiol. 12 , art. 616828