Publication Details |
Category | Text Publication |
Reference Category | Journals |
DOI | 10.1074/jbc.m611547200 |
Title (Primary) | A novel form of 6-phosphofructokinase. Identification and functional relevance of a third type of subunit in Pichia pastoris |
Author | Tanneberger, K.; Kirchberger, J.; Bär, J.; Schellenberger, W.; Rothemund, S.; Kamprad, M.; Otto, H.; Schöneberg, T.; Edelmann, A. |
Source Titel | Journal of Biological Chemistry |
Year | 2007 |
Department | ZELLTOX |
Volume | 282 |
Issue | 32 |
Page From | 23687 |
Page To | 23697 |
Language | englisch |
Abstract | Classically, 6-phosphofructokinases are homo- and hetero-oligomeric enzymes consisting of subunits and / subunits, respectively. Herein, we describe a new form of 6-phosphofructokinase (Pfk) present in several Pichia species, which is composed of three different types of subunit, , , and . The sequence of the subunit shows no similarity to classic Pfk subunits or to other known protein sequences. In-depth structural and functional studies revealed that the subunit is a constitutive component of Pfk from Pichia pastoris (PpPfk). Analyses of the purified PpPfk suggest a heterododecameric assembly from the three different subunits. Accordingly, it is the largest and most complex Pfk identified yet. Although, the subunit is not required for enzymatic activity, the subunit-deficient mutant displays a decreased growth on nutrient limitation and reduced cell flocculation when compared with the P. pastoris wild-type strain. Subsequent characterization of purified Pfks from wild-type and subunit-deficient strains revealed that the allosteric regulation of the PpPfk by ATP, fructose 2,6-bisphosphate, and AMP is fine-tuned by the subunit. Therefore, we suggest that the subunit contributes to adaptation of P. pastoris to energy resources. |
Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=2361 |
Tanneberger, K., Kirchberger, J., Bär, J., Schellenberger, W., Rothemund, S., Kamprad, M., Otto, H., Schöneberg, T., Edelmann, A. (2007): A novel form of 6-phosphofructokinase. Identification and functional relevance of a third type of subunit in Pichia pastoris J. Biol. Chem. 282 (32), 23687 - 23697 10.1074/jbc.m611547200 |