Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1371/journal.pgen.1008745
Licence creative commons licence
Title (Primary) Uptake of exogenous serine is important to maintain sphingolipid homeostasis in Saccharomyces cerevisiae
Author Esch, B.M.; Limar, S.; Bogdanowski, A.; Gournas, C.; More, T.; Sundag, C.; Walter, S.; Heinisch, J.J.; Ejsing, C.S.; André, B.; Fröhlich, F.
Source Titel PloS Genetics
Year 2020
Department OESA
Volume 16
Issue 8
Page From e1008745
Language englisch
Supplements https://ndownloader.figstatic.com/articles/12868928/versions/1
Abstract Sphingolipids are abundant and essential molecules in eukaryotes that have crucial functions as signaling molecules and as membrane components. Sphingolipid biosynthesis starts in the endoplasmic reticulum with the condensation of serine and palmitoyl-CoA. Sphingolipid biosynthesis is highly regulated to maintain sphingolipid homeostasis. Even though, serine is an essential component of the sphingolipid biosynthesis pathway, its role in maintaining sphingolipid homeostasis has not been precisely studied. Here we show that serine uptake is an important factor for the regulation of sphingolipid biosynthesis in Saccharomyces cerevisiae. Using genetic experiments, we find the broad-specificity amino acid permease Gnp1 to be important for serine uptake. We confirm these results with serine uptake assays in gnp1Δ cells. We further show that uptake of exogenous serine by Gnp1 is important to maintain cellular serine levels and observe a specific connection between serine uptake and the first step of sphingolipid biosynthesis. Using mass spectrometry-based flux analysis, we further observed imported serine as the main source for de novo sphingolipid biosynthesis. Our results demonstrate that yeast cells preferentially use the uptake of exogenous serine to regulate sphingolipid biosynthesis. Our study can also be a starting point to analyze the role of serine uptake in mammalian sphingolipid metabolism.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=23604
Esch, B.M., Limar, S., Bogdanowski, A., Gournas, C., More, T., Sundag, C., Walter, S., Heinisch, J.J., Ejsing, C.S., André, B., Fröhlich, F. (2020):
Uptake of exogenous serine is important to maintain sphingolipid homeostasis in Saccharomyces cerevisiae
PLoS Genet. 16 (8), e1008745 10.1371/journal.pgen.1008745