Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1128/mSphere.00016-20
Licence creative commons licence
Title (Primary) Inhibition of respiration of Candida albicans by small molecules increases phagocytosis efficacy by macrophages
Author Cui, S.; Li, M.; Hassan, R.Y.A.; Heintz-Buschart, A.; Wang, J.; Bilitewski, U.
Source Titel mSphere
Year 2020
Department BOOEK; iDiv
Volume 5
Page From e00016-20
Language englisch
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Keywords complex III; respiratory chain; antimycin A; phagocytosis; metabolism
Abstract Candida albicans adapts to various conditions in different body niches by regulating gene expression, protein synthesis, and metabolic pathways. These adaptive reactions not only allow survival but also influence the interaction with host cells, which is governed by the composition and structure of the fungal cell wall. Numerous studies had shown linkages between mitochondrial functionality, cell wall integrity and structure, and pathogenicity. Thus, we decided to inhibit single complexes of the respiratory chain of C. albicans and to analyze the resultant interaction with macrophages via their phagocytic activity. Remarkably, inhibition of the fungal bc1 complex by antimycin A increased phagocytosis, which correlated with an increased accessibility of β-glucans. To contribute to mechanistic insights, we performed metabolic studies, which highlighted significant changes in the abundance of constituents of the plasma membrane. Collectively, our results reinforce the strong linkage between fungal energy metabolism and other components of fungal physiology, which also determine the vulnerability to immune defense reactions.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=23469
Cui, S., Li, M., Hassan, R.Y.A., Heintz-Buschart, A., Wang, J., Bilitewski, U. (2020):
Inhibition of respiration of Candida albicans by small molecules increases phagocytosis efficacy by macrophages
mSphere 5 , e00016-20 10.1128/mSphere.00016-20