Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1007/s00216-020-02864-6 |
Licence  |
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| Title (Primary) | Direct sample introduction GC-MS/MS for quantification of organic chemicals in mammalian tissues and blood extracted with polymers without clean-up |
| Author | Baumer, A.; Escher, B.I.; Landmann, J.; Ulrich, N. |
| Source Titel | Analytical and Bioanalytical Chemistry |
| Year | 2020 |
| Department | AUC; ZELLTOX |
| Volume | 412 |
| Page From | 7295 |
| Page To | 7305 |
| Language | englisch |
| Supplements | https://static-content.springer.com/esm/art%3A10.1007%2Fs00216-020-02864-6/MediaObjects/216_2020_2864_MOESM1_ESM.pdf |
| Keywords | Mammalian tissues; Direct sample introduction; GC-MS/MS; Passive sampling; PDMS; Co-extracted matrix; Reduction of matrix effects |
| Abstract | Solvent extracts of mammalian tissues and blood contain a large amount of co-extracted matrix components, in particular lipids, which can adversely affect instrumental analysis. Clean-up typically degrades non-persistent chemicals. Alternatively, passive sampling with the polymer polydimethylsiloxane (PDMS) has been used for a comprehensive extraction from tissue without altering the mixture composition. Despite a smaller fraction of matrix being co-extracted by PDMS than by solvent extraction, direct analysis of PDMS extracts was only possible with direct sample introduction (DSI) GC-MS/MS, which prevented co-extracted matrix components entering the system. Limits of quantitation (LOQ) ranged from 4 to 20 pg μL−1 ethyl acetate (PDMS extract) for pesticides and persistent organic pollutants (POPs). The group of organophosphorus flame retardants showed higher LOQs up to 107 pg μL−1 due to sorption to active sites at the injection system. Intraday precision ranged between 1 and 10%, while the range of interday precision was between 1 and 18% depending on the analyte. The method was developed using pork liver, brain, and fat as well as blood and was then applied to analyze human post-mortem tissues where polychlorinated biphenyls (PCBs) as well as dichlorodiphenyltrichloroethane (DDT) and DDT metabolites were detected. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=23465 |
| Baumer, A., Escher, B.I., Landmann, J., Ulrich, N. (2020): Direct sample introduction GC-MS/MS for quantification of organic chemicals in mammalian tissues and blood extracted with polymers without clean-up Anal. Bioanal. Chem. 412 , 7295 - 7305 10.1007/s00216-020-02864-6 |
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