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Reference Category Reports
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Title (Primary) MRMS powered single cell metabolomics – Quantification of picogram amounts of a biocatalytic product from few living cells
Author Lohse, M.; Dusny, C.; Kaesler, J.; Lechtenfeld, O.J.;
Journal Bruker Application Note
Year 2020
Department ANA; SOMA;
Volume 05-2020, MRMS-70, 1877441
Page To 6
Language englisch;
Keywords solariX; single cell analysis; metabolomics; MRMS
Abstract Probing the metabolic state of living microbial cells via their secreted metabolic products is highly challenging as it requires measures to maintain the cellular integrity and to collect the analytes. This application note demonstrates how direct infusion magnetic resonance mass spectrometry (DI-MRMS) is able to quantify lysine produced by a few intact microbial cells. The high mass resolution and sensitivity of MRMS in combination with a reliable sample introduction and transfer is a helpful tool for assessing cellular processes.
ID 23445
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=23445
Lohse, M., Dusny, C., Kaesler, J., Lechtenfeld, O.J. (2020):
MRMS powered single cell metabolomics – Quantification of picogram amounts of a biocatalytic product from few living cells
Bruker Application Note 05-2020, MRMS-70, 1877441
Bruker Daltonics, 6 pp.