Publication Details |
| Category | Text Publication |
| Reference Category | Reports |
| DOI | 10.5281/zenodo.3947765 |
Licence  |
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| Title (Primary) | MRMS powered single cell metabolomics – Quantification of picogram amounts of a biocatalytic product from few living cells |
| Author | Lohse, M.; Dusny, C.; Kaesler, J.; Lechtenfeld, O.J.
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| Year | 2020 |
| Department | ANA; SOMA |
| Language | englisch |
| Keywords | solariX; single cell analysis; metabolomics; MRMS |
| Abstract | Probing the metabolic state of living microbial cells via their secreted metabolic products is highly challenging as it requires measures to maintain the cellular integrity and to collect the analytes. This application note demonstrates how direct infusion magnetic resonance mass spectrometry (DI-MRMS) is able to quantify lysine produced by a few intact microbial cells. The high mass resolution and sensitivity of MRMS in combination with a reliable sample introduction and transfer is a helpful tool for assessing cellular processes. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=23445 |
| Lohse, M., Dusny, C., Kaesler, J., Lechtenfeld, O.J. (2020): MRMS powered single cell metabolomics – Quantification of picogram amounts of a biocatalytic product from few living cells Bruker Daltonics, 10.5281/zenodo.3947765 |
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