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Title (Primary) Label‐free four‐dimensional visualization of anaerobically growing electroactive biofilms
Author Koch, C.; Kuchenbuch, A.; Marosvölgyi, M.; Weisshart, K.; Harnisch, F.;
Journal Cytometry Part A
Year 2020
Department UMB;
Volume 97
Issue 7
Language englisch;
POF III (all) R31;
Supplements https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fcyto.a.24169&file=cytoa24169-sup-0001-supinfo.docx
https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fcyto.a.24169&file=cytoa24169-sup-0001-VideoS1.mp4
https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fcyto.a.24169&file=cytoa24169-sup-0002-VideoS2.mp4
Keywords Light sheet fluorescence microscopy; Geobacter; live cell imaging; electroactive biofilm growth
Abstract Light sheet fluorescence microscopy (LSFM) allows non‐destructive, label‐free and in vivo imaging of large specimen, even at non‐transparent surfaces. We show that LSFM can be applied for label‐free analyses of prokaryotes on the example of electroactive biofilms. Biofilm growth is linked to the production of current serving as measure of metabolic activity in vivo by monitoring with high spatial and temporal resolution. After 35 h of exponential growth a homogeneous biofilm with a thickness of 9 μm was formed. This was followed by a stratification of the biofilm including formation of 3D structures over the next 100 h. Light reflection was sufficient to visualize the biofilm structure and development over time and the terminal morphology was confirmed using fluorescence staining. This proof of concept on using LSFM for investigation of biofilms opens the door for its application in the entire field of microbial ecology.
ID 23255
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=23255
Koch, C., Kuchenbuch, A., Marosvölgyi, M., Weisshart, K., Harnisch, F. (2020):
Label‐free four‐dimensional visualization of anaerobically growing electroactive biofilms
Cytom. Part A 97 (7), 737 - 741