Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1098/rstb.2019.0645
Document author version
Title (Primary) Fatty acid bioconversion in harpacticoid copepods in a changing environment: a transcriptomic approach
Author Boyen, J.; Fink, P. ORCID logo ; Mensens, C.; Hablützel, P.I.; De Troch, M.
Source Titel Philosophical Transactions of the Royal Society B-Biological Sciences
Year 2020
Department ASAM; FLOEK
Volume 375
Page From art. 20190645
Language englisch
Keywords harpacticoid copepods, fatty acid metabolism, transcriptomics, global warming
Abstract By 2100, global warming is predicted to significantly reduce the capacity of marine primary producers for long-chain polyunsaturated fatty acid (LC-PUFA) synthesis. Primary consumers such as harpacticoid copepods (Crustacea) might mitigate the resulting adverse effects on the food web by increased LC-PUFA bioconversion. Here, we present a high-quality de novo transcriptome assembly of the copepod Platychelipus littoralis, exposed to changes in both temperature (+3°C) and dietary LC-PUFA availability. Using this transcriptome, we detected multiple transcripts putatively encoding for LC-PUFA-bioconverting front-end fatty acid desaturases and elongases, and performed phylogenetic analyses to identify their relationship with sequences of other (crustacean) taxa. While temperature affected the absolute fatty acid concentrations in copepods, LC-PUFA levels remained unaltered even when copepods were fed a LC-PUFA-deficient diet. While this suggests plasticity of LC-PUFA bioconversion within P. littoralis, none of the putative front-end desaturase or elongase transcripts were differentially expressed under the applied treatments. Nevertheless, the transcriptome presented here provides a sound basis for future ecophysiological research on harpacticoid copepods.
Persistent UFZ Identifier
Boyen, J., Fink, P., Mensens, C., Hablützel, P.I., De Troch, M. (2020):
Fatty acid bioconversion in harpacticoid copepods in a changing environment: a transcriptomic approach
Philos. Trans. R. Soc. B-Biol. Sci. 375 , art. 20190645 10.1098/rstb.2019.0645