Publication Details

Category Text Publication
Reference Category Journals
DOI 10.3390/nano10061021
Licence creative commons licence
Title (Primary) Attachment efficiency of nanomaterials to algae as an important criterion for ecotoxicity and grouping
Author Hund-Rinke, K.; Sinram, T.; Schlich, K.; Nickel, C.; Dickehut, H.P.; Schmidt, M.; Kühnel, D.
Journal Nanomaterials
Year 2020
Volume 10
Issue 6
Page From art. 1021
Language englisch
Keywords nanotoxicology; European Chemicals Agency (ECHA), ecotoxicology; nanoparticles; aggregation; Raphidocelis subcapitata
UFZ wide themes ProVIS;
Abstract Engineered nanomaterials (ENMs) based on CeO2 and TiO2 differ in their effects on the unicellular green alga Raphidocelis subcapitata but these effects do not reflect the physicochemical parameters that characterize such materials in water and other test media. To determine whether interactions with algae can predict the ecotoxicity of ENMs, we studied the attachment of model compounds (three subtypes of CeO2 and five subtypes of TiO2) to algal cells by light microscopy and electron microscopy. We correlated our observations with EC50 values determined in growth inhibition assays carried out according to the Organisation for Economic Co-operation and Development (OECD) test guideline 201. Light microscopy revealed distinct patterns of ENM attachment to algal cells according to the type of compound, with stronger interactions leading to greater toxicity. This was confirmed by electron microscopy, which allowed the quantitative assessment of particle attachment. Our results indicate that algal extracellular polymeric substances play an important role in the attachment of ENMs, influencing the formation of agglomerates. The attachment parameters in short-term tests predicted the toxicity of CeO2 and TiO2 ENMs and can be considered as a valuable tool for the identification of sets of similar nanoforms as requested by the European Chemicals Agency in the context of grouping and read-across.
Persistent UFZ Identifier
Hund-Rinke, K., Sinram, T., Schlich, K., Nickel, C., Dickehut, H.P., Schmidt, M., Kühnel, D. (2020):
Attachment efficiency of nanomaterials to algae as an important criterion for ecotoxicity and grouping
Nanomaterials 10 (6), art. 1021