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Title (Primary) Water column anammox and denitrification in a temperate permanently stratified lake (Lake Rassnitzer, Germany)
Author Hamersley, M.R.; Woebken, D.; Boehrer, B.; Schultze, M.; Lavik, G.; Kuypers, M.M.M.;
Journal Systematic and Applied Microbiology
Year 2009
Department SEEFO;
Volume 32
Issue 8
Language englisch;
Keywords Anammox; Denitrification; Water column; Meromictic; Lake; CARD-FISH
Abstract We studied microbial N2 production via anammox and denitrification in the anoxic water column of a restored mining pit lake in Germany over an annual cycle. We obtained high-resolution hydrochemical profiles using a continuous pumping sampler. Lake Rassnitzer is permanently stratified at ca. 29 m depth, entraining anoxic water below a saline density gradient. Mixed-layer nitrate concentrations averaged ca. 200 µmol L-1, but decreased to zero in the anoxic bottom waters. In contrast, ammonium was <5 µmol L-1 in the mixed layer but increased in the anoxic waters to ca. 600 µmol L-1 near the sediments. In January and October, 15N tracer measurements detected anammox activity (maximum 504 nmol N2 L-1 d-1 in 15NH4+-amended incubations), but no denitrification. In contrast, in May, N2 production was dominated by denitrification (maximum 74 nmol N2 L-1 d-1). Anammox activity in May was significantly lower than in October, as characterized by anammox rates (maximum 6 vs. 16 nmol N2 L-1 d-1 in incubations with 15NO3-), as well as relative and absolute anammox bacterial cell abundances (0.56% vs. 0.98% of all bacteria, and 2.7×104 vs. 5.2×104 anammox cells mL-1, respectively) (quantified by catalyzed reporter deposition-fluorescence in situ hybridization (CARD-FISH) with anammox bacteria-specific probes). Anammox bacterial diversity was investigated with anammox bacteria-specific 16S rRNA gene clone libraries. The majority of anammox bacterial sequences were related to the widespread Candidatus Scalindua sorokinii/brodae cluster. However, we also found sequences related to Candidatus S. wagneri and Candidatus Brocadia fulgida, which suggests a high anammox bacterial diversity in this lake comparable with estuarine sediments.
ID 231
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Hamersley, M.R., Woebken, D., Boehrer, B., Schultze, M., Lavik, G., Kuypers, M.M.M. (2009):
Water column anammox and denitrification in a temperate permanently stratified lake (Lake Rassnitzer, Germany)
Syst. Appl. Microbiol. 32 (8), 571 - 582