Publication Details

Category Text Publication
Reference Category Journals
DOI 10.3390/microorganisms8040614
Licence creative commons licence
Title (Primary) Microbial resource management for ex situ biomethanation of hydrogen at alkaline pH
Author Logroño, W.; Popp, D.; Kleinsteuber, S. ORCID logo ; Sträuber, H.; Harms, H.; Nikolausz, M.
Source Titel Microorganisms
Year 2020
Department UMB
Volume 8
Page From art. 614
Language englisch
Keywords power-to-gas; energy storage; biogas upgrading; biomethane; formate; hydrogenotrophic methanogenesis; homoacetogenesis; Methanobacterium; Methanoculleus
Abstract Biomethanation is a promising solution to convertH2 (produced fromsurplus electricity) and CO2 to CH4 by using hydrogenotrophic methanogens. In ex situ biomethanation with mixed cultures, homoacetogens and methanogens compete for H2/CO2. We enriched a hydrogenotrophic microbiota on CO2 and H2 as sole carbon and energy sources, respectively, to investigate these competing reactions. The microbial community structure and dynamics of bacteria and methanogenic archaea were evaluated through 16S rRNA and mcrA gene amplicon sequencing, respectively. Hydrogenotrophic methanogens and homoacetogens were enriched, as acetate was concomitantly produced alongside CH4. By controlling the media composition, especially changing the reducing agent, the formation of acetate was lowered and grid quality CH4 (≥97%) was obtained. Formate was identified as an intermediate that was produced and consumed during the bioprocess. Stirring intensities ≥ 1000 rpm were detrimental, probably due to shear force stress. The predominating methanogens belonged to the genera Methanobacterium and Methanoculleus. The bacterial community was dominated by
Lutispora. The methanogenic community was stable, whereas the bacterial community was more dynamic. Our results suggest that hydrogenotrophic communities can be steered towards the selective production of CH4 from H2/CO2 by adapting the media composition, the reducing agent and the stirring intensity.
Persistent UFZ Identifier
Logroño, W., Popp, D., Kleinsteuber, S., Sträuber, H., Harms, H., Nikolausz, M. (2020):
Microbial resource management for ex situ biomethanation of hydrogen at alkaline pH
Microorganisms 8 , art. 614 10.3390/microorganisms8040614