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Title (Primary) Continuous synthesis and excretion of the compatible solute ectoine by a transgenic, nonhalophilic bacterium
Author Schubert, T.; Maskow, T.; Benndorf, D.; Harms, H.; Breuer, U.;
Journal Applied and Environmental Microbiology
Year 2007
Department UMB; PROTEOM;
Volume 73
Issue 10
Language englisch;
Abstract The compatible solute 1,4,5,6-tetrahydro-2-methyl-4-pyrimidinecarboxylic acid (ectoine) acts in microorganisms as an osmotic counterweight against halostress and has attracted commercial attention as a protecting agent. Its production and application are restricted by the drawbacks of the discontinuous harvesting procedure involving salt shocks, which reduces volumetric yield, increases reactor corrosion, and complicates downstream processing. In order to synthesize ectoine continuously in less-aggressive media, we introduced the ectoine genes ectABC of the halophilic bacterium Chromohalobacter salexigens into an Escherichia coli strain using the expression vector pASK-IBA7. Under the control of a tet promoter, the transgenic E. coli synthesized 6 g liter-1 ectoine with a space-time yield of 40 mg liter-1 h-1, with the vast majority of the ectoine being excreted.
ID 2284
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=2284
Schubert, T., Maskow, T., Benndorf, D., Harms, H., Breuer, U. (2007):
Continuous synthesis and excretion of the compatible solute ectoine by a transgenic, nonhalophilic bacterium
Appl. Environ. Microb. 73 (10), 3343 - 3347