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Title (Primary) Response‐surface‐optimized and scaled‐up microbial electrosynthesis of chiral alcohols
Author Mayr, J.C.; Rosa, L.F.M.; Klinger, N.; Grosch, J.-H.; Harnisch, F.; Spiess, A.C.;
Journal ChemSusChem
Year 2020
Department UMB;
Volume 13
Issue 7
Language englisch;
POF III (all) R31; T15;
Keywords biocatalysis; cofactor regeneration; electrobiotechnology; enantioselectivity; microbial electrosynthesis
Abstract A whole variety of enzymes can be easily incorporated and overexpressed within Escherichia coli cells via plasmids, making it an ideal chassis for bioelectrosynthesis. We recently demonstrated microbial electrosynthesis (MES) of chiral alcohols using genetically modified E. coli with plasmid‐incorporated and overexpressed enzymes and methyl viologen as mediator for electron transfer. Here, this model system, using NADPH‐dependent alcohol dehydrogenase from Lactobacillus brevis to convert acetophenone to ( R )‐1‐phenylethanol, was assessed by a Design of Experiment (DoE) approach. Process optimization was achieved with a 2.4‐fold increased yield of 94±7%, a 3.9‐fold increased reaction rate of 324±67 μM h ‐1 , and a coulombic efficiency of up to 68±7%, while maintaining an excellent enantioselectivity of >99%. Subsequent scale‐up to 1 L using electrobioreactors under batch and fed‐batch conditions increased the titer of ( R )‐1‐phenylethanol to 12.8±2.0 mM and paves the way to further develop E. coli into a universal chassis for MES in a standard biotechnological process environment.
ID 22824
Persistent UFZ Identifier
Mayr, J.C., Rosa, L.F.M., Klinger, N., Grosch, J.-H., Harnisch, F., Spiess, A.C. (2020):
Response‐surface‐optimized and scaled‐up microbial electrosynthesis of chiral alcohols
ChemSusChem 13 (7), 1808 - 1816