|DOI / URL||link|
|Creative Commons Licence|
|Title (Primary)||Gastric bypass surgery in a rat model alters the community structure and functional composition of the intestinal microbiota independently of weight loss|
|Author||Haange, S.-B.; Jehmlich, N.; Krügel, U.; Hintschich, C.; Wehrmann, D.; Hankir, M.; Seyfried, F.; Froment, J.; Hübschmann, T.; Müller, S.; Wissenbach, D.K.; Kang, K.H.; Buettner, C.; Panagiotou, G.; Noll, M.; Rolle-Kampczyk, U.; Fenske, W.; von Bergen, M.;|
|POF III (all)||F11;|
Roux-en-Y gastric bypass (RYGB) surgery is a last-resort treatment to induce substantial and sustained weight loss in cases of severe obesity. This anatomical rearrangement affects the intestinal microbiota, but so far, little information is available on how it interferes with microbial functionality and microbial-host interactions independently of weight loss.
A rat model was employed where the RYGB-surgery cohort is compared to sham-operated controls which were kept at a matched body weight by food restriction. We investigated the microbial taxonomy and functional activity using 16S rRNA amplicon gene sequencing, metaproteomics, and metabolomics on samples collected from theileum, the cecum, and the colon, and separately analysed the lumen and mucus-associated microbiota.
Altered gut architecture in RYGB increased the relative occurrence of Actinobacteria, especially Bifidobacteriaceae and Proteobacteria, while in general, Firmicutes were decreased although Streptococcaceae and Clostridium perfringens were observed at relative higher abundances independent of weight loss. A decrease of conjugated and secondary bile acids was observed in the RYGB-gut lumen. The arginine biosynthesis pathway in the microbiota was altered, as indicated by the changes in the abundance of upstream metabolites and enzymes, resulting in lower levels of arginine and higher levels of aspartate in the colon after RYGB.
|Persistent UFZ Identifier||https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=22813|
|Haange, S.-B., Jehmlich, N., Krügel, U., Hintschich, C., Wehrmann, D., Hankir, M., Seyfried, F., Froment, J., Hübschmann, T., Müller, S., Wissenbach, D.K., Kang, K.H., Buettner, C., Panagiotou, G., Noll, M., Rolle-Kampczyk, U., Fenske, W., von Bergen, M. (2020):
Gastric bypass surgery in a rat model alters the community structure and functional composition of the intestinal microbiota independently of weight loss
Microbiome 8 , art. 13