Publication Details
Category Text Publication
Reference Category Journals
DOI 10.1002/rcm.8668
Licence creative commons licence
Title (Primary) Quantification of glyphosate and AMPA from microbiome reactor fluids
Author Fritz-Wallace, K.; Engelmann, B.; Krause, J.L.; Schäpe, S.S.; Pöppe, J.; Herberth, G. ORCID logo ; Rösler, U.; Jehmlich, N. ORCID logo ; von Bergen, M.; Rolle-Kampczyk, U.
Source Titel Rapid Communications in Mass Spectrometry
Year 2020
Department IMMU; MOLSYB
Volume 34
Issue 7
Page From e8668
Language englisch
Supplements https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Frcm.8668&file=rcm8668-sup-0001-Supplementary+materials.docx
https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Frcm.8668&file=rcm8668-sup-0002-Extraction+of+standard+curve.tif
Keywords Glyphosate; AMPA; LC/MS/MS; microbiome bioreactor; Roundup
Abstract RATIONALE
Glyphosate is one of the most widely used herbicides and it is suspected to affect the intestinal microbiota through inhibition of aromatic amino acid synthesis via the shikimate pathway. In vitro microbiome bioreactors are increasingly used as model systems to investigate effects on the intestinal microbiota and consequently methods for the quantitation of glyphosate and its degradation product AMPA in microbiome model systems are required.
METHODS
An optimized protocol enables the analysis of both glyphosate and AMPA by simple extraction with methanol:acetonitrile:water (2:3:1) without further enrichment steps. Glyphosate and AMPA are separated by liquid chromatography on an amide column and identified and quantified with a targeted MS/MS method on a QTRAP 5500 system (AB Sciex).
RESULTS
Our method has a limit of detection (LOD) in extracted water samples of < 2 ng/mL for both glyphosate and AMPA. In complex intestinal medium the LOD is 2 ng/mL and 5 ng/mL for glyphosate and AMPA, respectively. These LODs allow for measurement at exposure‐relevant concentrations. Glyphosate levels in a bioreactor model of porcine colon were determined and consequently it was verified whether AMPA was produced by porcine gut microbiota.
CONCLUSIONS
The method presented here allows quantitation of glyphosate and AMPA in complex bioreactor fluids and thus enables studies on the impact of glyphosate and its metabolism on the intestinal microbiota. In addition, the extraction protocol is compatible with an untargeted metabolomics analysis, thus allowing other perturbations caused by glyphosate in the same sample to be looked for.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=22700
Fritz-Wallace, K., Engelmann, B., Krause, J.L., Schäpe, S.S., Pöppe, J., Herberth, G., Rösler, U., Jehmlich, N., von Bergen, M., Rolle-Kampczyk, U. (2020):
Quantification of glyphosate and AMPA from microbiome reactor fluids
Rapid Commun. Mass Spectrom. 34 (7), e8668 10.1002/rcm.8668