Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1093/nar/gkz833 |
Licence  |
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| Title (Primary) | Improved annotation of protein-coding genes boundaries in metazoan mitochondrial genomes |
| Author | Donath, A.; Jühling, F.; Al-Arab, M.; Bernhart, S.H.; Reinhardt, F.; Stadler, P.F.; Middendorf, M.; Bernt, M.
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| Source Titel | Nucleic Acids Research |
| Year | 2019 |
| Department | MOLSYB |
| Volume | 47 |
| Issue | 20 |
| Page From | 10543 |
| Page To | 10552 |
| Language | englisch |
| Supplements | https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/nar/47/20/10.1093_nar_gkz833/1/gkz833_supplemental_file.pdf?Expires=1581166712&Signature=B9G5y5lxvcOfPczVe7x-zF6-sqb5UBTNKJUkUOnarM2ZyRmkPmCK2nS5V-acpbe0Tans0~9UrRG1hdYuxn~5ufZWkzjee7B-yxyIt~KU9dE0UW3TNko60ziaOAoSBPoCSNyqWRH1zvYyrPJI7FtcjOwNA~6o0qL6I7hbIugv9SjZTi6~PFk6T-oC6IAi5JhDgyFpTm05MVQTdvYGVffH8EIEmVf102gRmCm6YhOBTRW9Mc9BuhuvBgobhAbQ4B0gFzuroLn8aCYs4aB5YZSs5HhNVvob9LswBcGhUs9KUSFJD1WQ6BABTy8xk84GlALjrPjJy7i4j-vRIu2~pUhvvw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA |
| Abstract | With the rapid increase of sequenced metazoan mitochondrial genomes, a detailed manual annotation is becoming more and more infeasible. While it is easy to identify the approximate location of protein-coding genes within mitogenomes, the peculiar processing of mitochondrial transcripts, however, makes the determination of precise gene boundaries a surprisingly difficult problem. We have analyzed the properties of annotated start and stop codon positions in detail, and use the inferred patterns to devise a new method for predicting gene boundaries in de novo annotations. Our method benefits from empirically observed prevalances of start/stop codons and gene lengths, and considers the dependence of these features on variations of genetic codes. Albeit not being perfect, our new approach yields a drastic improvement in the accuracy of gene boundaries and upgrades the mitochondrial genome annotation server MITOS to an even more sophisticated tool for fully automatic annotation of metazoan mitochondrial genomes. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=22589 |
| Donath, A., Jühling, F., Al-Arab, M., Bernhart, S.H., Reinhardt, F., Stadler, P.F., Middendorf, M., Bernt, M. (2019): Improved annotation of protein-coding genes boundaries in metazoan mitochondrial genomes Nucleic Acids Res. 47 (20), 10543 - 10552 10.1093/nar/gkz833 |
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