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DOI 10.1007/s11356-019-06584-y
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Title (Primary) Anaerobic degradation of 2,4,5-trichlorophenoxyacetic acid by enrichment cultures from freshwater sediments
Author Al-Fathi, H.; Koch, M.; Lorenz, W.G.; Lechner, U.
Source Titel Environmental Science and Pollution Research
Year 2019
Department UMB
Volume 26
Issue 33
Page From 34459
Page To 34467
Language englisch
Keywords 2,4,5-T Chlorophenoxyacetic acid herbicides; Anaerobic degradation; Dehalogenation; Anoxic sediment; Desulfitobacterium hafniense; Dehalococcoides mccartyi
Abstract The anaerobic biodegradation of 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) was investigated using enrichment cultures from freshwater sediments at two different sites in the region of Halle, central Germany. 2,4,5-T and different organic acids or hydrogen were added as possible electron acceptor and electron donors, respectively. The primary enrichment cultures from Saale river sediment completely degraded 2,4,5-T to 3-chlorophenol (3-CP) (major product) and 3,4-dichlorophenol (3,4-DCP) during a 28-day incubation period. Subcultures showed ether cleavage of 2,4,5-T to 2,4,5-trichlorophenol and its stoichiometric dechlorination to 3-CP only in the presence of butyrate. In contrast, the primary enrichment culture from sediment of Posthorn pond dechlorinated 2,4,5-T to 2,5-dichlorophenoxyacetic acid (2,5-D), which, in the presence of butyrate, was degraded further to products such as 3,4-DCP, 2,5-DCP, and 3CP, indicating ether cleaving activities and subsequent dechlorination steps. Experiments with pure cultures of Dehalococcoides mccartyi and Desulfitobacterium hafniense demonstrated their specific dechlorination steps within the overall 2,4,5-T degradation pathways. The results indicate that the route and efficiency of anaerobic 2,4,5-T degradation in the environment depend heavily on the microorganisms present and the availability of slowly fermentable organic compounds.
Persistent UFZ Identifier
Al-Fathi, H., Koch, M., Lorenz, W.G., Lechner, U. (2019):
Anaerobic degradation of 2,4,5-trichlorophenoxyacetic acid by enrichment cultures from freshwater sediments
Environ. Sci. Pollut. Res. 26 (33), 34459 - 34467 10.1007/s11356-019-06584-y