Publication Details

Reference Category Journals
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Title (Primary) Pseudomonas spp. are key players in agricultural biogas substrate degradation
Author Buettner, C.; von Bergen, M.; Jehmlich, N.; Noll, M.;
Journal Scientific Reports
Year 2019
Department MOLSYB;
Volume 9
Language englisch;
POF III (all) F11;
Data links https://www.ebi.ac.uk/pride/archive/projects/PXD014605
Supplements https://static-content.springer.com/esm/art%3A10.1038%2Fs41598-019-49313-8/MediaObjects/41598_2019_49313_MOESM1_ESM.docx
https://static-content.springer.com/esm/art%3A10.1038%2Fs41598-019-49313-8/MediaObjects/41598_2019_49313_MOESM2_ESM.xlsx
https://static-content.springer.com/esm/art%3A10.1038%2Fs41598-019-49313-8/MediaObjects/41598_2019_49313_MOESM3_ESM.xlsx
https://static-content.springer.com/esm/art%3A10.1038%2Fs41598-019-49313-8/MediaObjects/41598_2019_49313_MOESM4_ESM.xlsx
Abstract Anaerobic degradation (AD) of heterogeneous agricultural substrates is a complex process involving a diverse microbial community. While microbial community composition of a variety of biogas plants (BPs) is well described, little is known about metabolic processes and microbial interaction patterns. Here, we analyzed 16 large-scale BPs using metaproteomics. All metabolic steps of AD were observed in the metaproteome, and multivariate analyses indicated that they were shaped by temperature, pH, volatile fatty acid content and substrate types. Biogas plants could be subdivided into hydrogenotrophic, acetoclastic or a mixture of both methanogenic pathways based on their process parameters, taxonomic and functional metaproteome. Network analyses showed large differences in metabolic and microbial interaction patterns. Both, number of interactions and interaction partners were highly dependent on the prevalent methanogenic pathway for most species. Nevertheless, we observed a highly conserved metabolism of different abundant Pseudomonas spp. for all BPs indicating a key role during AD in carbohydrate hydrolysis irrespectively of variabilities in substrate input and process parameters. Thus, Pseudomonas spp. are of high importance for robust and versatile AD food webs, which highlight a large variety of downstream metabolic processes for their respective methanogenic pathways.
ID 22243
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=22243
Buettner, C., von Bergen, M., Jehmlich, N., Noll, M. (2019):
Pseudomonas spp. are key players in agricultural biogas substrate degradation
Sci. Rep. 9 , 12871