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Title (Primary) Biocatalyst design for 1,2-propanediol synthesis from CO2 employing the photoautotrophic strain Synechocystis sp. PCC 6803
Author David, C.;
Journal PhD Dissertation
Year 2019
Department SOMA;
Volume 4/2019
Language englisch;
POF III (all) R61;
Keywords Photoautotropher Mikroorganismus; Biokatalyse; CO2; Biocatalysis; Photoautotrophic microogranism; Stoffwechsel; Metabolism
UFZ inventory Leipzig, Bibliothek, Reportsammlung, 00534215, 19-0322 F/E
Abstract The application of photoautotrophic microorganisms in biotechnology enables the development of next-generation microbial cell factories, which utilize sunlight to (re-)activate carbon dioxide. This enables the cells to provide carbon skeletons for the production of chemical feedstock and biofuels without the need for an organic carbon and energy source. However, up to now, the production rates and product titers achieved with these cell factories are low and far away from an economically feasible process. Further progress has to be made in biocatalyst and process engineering to increase the understanding and fully exploit the capacities of these biocatalysts. This work focused on the development of a photoautotrophic whole-cell biocatalyst for the production of 1,2-propanediol (propylene glycol). Particular focus was placed on the interconnection between the accumulation of internal storage compounds and the influence on the production capacity of the synthetic pathway. A synthetic pathway comprising a methylglyoxal synthase, an aldehyde reductase, and an alcohol dehydrogenase was inserted into Synechocystis sp. PCC 6803 to convert dihydroxyacetone phosphate to methylglyoxal, which is subsequently reduced to acetol and then to 1,2-propanediol.
ID 22121
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=22121
David, C. (2019):
Biocatalyst design for 1,2-propanediol synthesis from CO2 employing the photoautotrophic strain Synechocystis sp. PCC 6803
Dissertation, TU Dresden
PhD Dissertation 4/2019
Helmholtz-Zentrum für Umweltforschung - UFZ, Leipzig, 149 pp.