Publication Details

Reference Category Journals
DOI / URL link
Creative Commons Licence creative commons licence
Title (Primary) Diffraction-limited axial scanning in thick biological tissue with an aberration-correcting adaptive lens
Author Philipp, K.; Lemke, F.; Scholz, S.; Wallrabe, U.; Wapler, M.C.; Koukourakis, N.; Czarske, J.W.
Journal Scientific Reports
Year 2019
Department BIOTOX
Volume 9
Page From art. 9532
Language englisch
Abstract Diffraction-limited deep focusing into biological tissue is challenging due to aberrations that lead to a broadening of the focal spot. The diffraction limit can be restored by employing aberration correction for example with a deformable mirror. However, this results in a bulky setup due to the required beam folding. We propose a bi-actuator adaptive lens that simultaneously enables axial scanning and the correction of specimen-induced spherical aberrations with a compact setup. Using the bi-actuator lens in a confocal microscope, we show diffraction-limited axial scanning up to 340 μm deep inside a phantom specimen. The application of this technique to in vivo measurements of zebrafish embryos with reporter-gene-driven fluorescence in a thyroid gland reveals substructures of the thyroid follicles, indicating that the bi-actuator adaptive lens is a meaningful supplement to the existing adaptive optics toolset.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=22023
Philipp, K., Lemke, F., Scholz, S., Wallrabe, U., Wapler, M.C., Koukourakis, N., Czarske, J.W. (2019):
Diffraction-limited axial scanning in thick biological tissue with an aberration-correcting adaptive lens
Sci. Rep. 9 , art. 9532