Publication Details

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Title (Primary) Incomplete cell disruption of resistant microbes
Author Starke, R.; Jehmlich, N.; Alfaro, T.; Dohnalkova, A.; Capek, P.; Bell, S.L.; Hofmockel, K.S.;
Journal Scientific Reports
Year 2019
Department MOLSYB;
Volume 9
Language englisch;
POF III (all) T41;
Supplements https://static-content.springer.com/esm/art%3A10.1038%2Fs41598-019-42188-9/MediaObjects/41598_2019_42188_MOESM1_ESM.docx
Abstract Biomolecules for OMIC analysis of microbial communities are commonly extracted by bead-beating or ultra-sonication, but both showed varying yields. In addition to that, different disruption pressures are necessary to lyse bacteria and fungi. However, the disruption efficiency and yields comparing bead-beating and ultra-sonication of different biological material have not yet been demonstrated. Here, we show that ultra-sonication in a bath transfers three times more energy than bead-beating over 10 min. TEM imaging revealed intact gram-positive bacterial and fungal cells whereas the gram-negative bacterial cells were destroyed beyond recognition after 10 min of ultra-sonication. DNA extraction using 10 min of bead-beating revealed higher yields for fungi but the extraction efficiency was at least three-fold lower considering its larger genome. By our critical viewpoint, we encourage the review of the commonly used extraction techniques as we provide evidence for a potential underrepresentation of resistant microbes, particularly fungi, in ecological studies.
ID 21790
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=21790
Starke, R., Jehmlich, N., Alfaro, T., Dohnalkova, A., Capek, P., Bell, S.L., Hofmockel, K.S. (2019):
Incomplete cell disruption of resistant microbes
Sci. Rep. 9 , art. 5618