Publication Details

Reference Category Journals
DOI / URL link
Title (Primary) Investigation of architecture development and phosphate distribution in Chlorella biofilm by complementary microscopy techniques
Author Moreno Osorio, J.H.; Benettoni, P.; Schmidt, M.; Stryhanyuk, H.; Schmitt-Jansen, M.; Pinto, G.; Pollio, A.; Frunzo, L.; Lens, P.N.L.; Richnow, H.H.; Esposito, G.; Musat, N.;
Journal FEMS Microbiology Ecology
Year 2019
Department ISOBIO; BIOTOX;
Volume 95
Issue 4
Language englisch;
POF III (all) T15;
Supplements https://academic.oup.com/femsec/article/95/4/fiz029/5372415#supplementary-data
Keywords Chlorella species; phosphorous distribution; chemical imaging; multimodal imaging; biofilm development; HIM; ToF-SIMS
Abstract

Microalgae biofilms may play an important role in the mitigation and prevention of eutrophication caused by domestic, agricultural and industrial wastewater effluents. Despite their potential, the biofilm development and role in nutrient removal are not well understood. Its clarification requires comprehensive studies of the complex three-dimensional architecture of the biofilm. In this study, we established a multimodal imaging approach to provide key information regarding architecture development and nutrient distribution in the biofilm of two green algae organisms: Chlorella pyrenoidosa and Chlorella vulgaris. Helium ion microscopy (HIM), scanning electron microscopy coupled with energy dispersive X-ray analysis (SEM-EDX) and time-of-flight secondary ion mass spectrometry (ToF-SIMS) were employed for i) elucidation of spatial arrangement, ii) elemental mapping and iii) 3D chemical imaging of the biofilm. The fine structure of the algal biofilm was resolved by HIM, the evidence of the accumulation of phosphate in hot spots was provided by SEM-EDX and the localization of phosphate oxides granules throughout the whole sample was clarified by ToF-SIMS. The reported results shed light on the phosphorus distribution during Chlorella's biofilm formation and highlight the potential of such correlative approach to solve fundamental question in algal biotechnology research.

ID 21741
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=21741
Moreno Osorio, J.H., Benettoni, P., Schmidt, M., Stryhanyuk, H., Schmitt-Jansen, M., Pinto, G., Pollio, A., Frunzo, L., Lens, P.N.L., Richnow, H.H., Esposito, G., Musat, N. (2019):
Investigation of architecture development and phosphate distribution in Chlorella biofilm by complementary microscopy techniques
FEMS Microbiol. Ecol. 95 (4), fiz029