Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1007/s00216-018-1471-2
Licence creative commons licence
Title (Primary) Elemental imaging (LA-ICP-MS) of zebrafish embryos to study the toxicokinetics of the acetylcholinesterase inhibitor naled
Author Halbach, K.; Wagner, S.; Scholz, S.; Luckenbach, T.; Reemtsma, T.
Journal Analytical and Bioanalytical Chemistry
Year 2019
Department BIOTOX; ANA
Volume 411
Issue 3
Page From 617
Page To 627
Language englisch
Keywords Elemental distribution; Enzyme inhibition; Quantitative bioimaging; Biotransformation; Bioaccumulation; Reactive toxicity
UFZ wide themes ProVIS;
Abstract The zebrafish embryo is an important model in ecotoxicology but the spatial distribution of chemicals and the relation to observed effects is not well understood. Quantitative imaging can help to gain insights into the distribution of chemicals in the zebrafish embryo. Laser ablation inductively coupled plasma-mass spectrometry (LA-ICP-MS) is used to quantify the uptake and the uptake kinetics of the bromine (Br) containing organophosphate naled (DibromĀ®, dimethyl-1,2-dibromo-2,2-dichloroethylphosphate) and its distribution in zebrafish embryos using Br as the marker element. During exposure, the Br amounts increase in the embryos parallel to the irreversible inhibition of the acetylcholinesterase (AChE). The final amount of Br in the embryo (545 pmol/embryo) corresponds to a 280-fold enrichment of naled from the exposure solution. However, LC-MS/MS analyses showed that the internal concentration of naled remained below the LOD (7.8 fmol/embryo); also the concentration of its known transformation product dichlorvos remained low (0.85 to 2.8 pmol/embryo). These findings indicate the high reactivity and high transformation rate of naled to other products than dichlorvos. 12C normalized intensity distributions of Br in the zebrafish embryo showed an enrichment of Br in its head region. Kernel density estimates of the LA-ICP-MS data were calculated and outline the high reproducibility between replicated and the shift in the Br distribution during exposure. The Br enrichment indicates a preferential debromination or direct covalent reaction of naled with AChE in this region.
Persistent UFZ Identifier
Halbach, K., Wagner, S., Scholz, S., Luckenbach, T., Reemtsma, T. (2019):
Elemental imaging (LA-ICP-MS) of zebrafish embryos to study the toxicokinetics of the acetylcholinesterase inhibitor naled
Anal. Bioanal. Chem. 411 (3), 617 - 627