Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/j.jhazmat.2018.11.054
Document author version
Title (Primary) Optimizing the metribuzin degrading potential of a novel bacterial consortium based on Taguchi design of experiment
Author Wahla, A.Q.; Iqbal, S.; Anwar, S.; Firdous, S.; Müller, J.A.
Source Titel Journal of Hazardous Materials
Year 2019
Department UBT
Volume 366
Page From 1
Page To 9
Language englisch
Keywords Metribuzin; Taguchi DOE; diketo-metribuzin (DK); desamino-metribuzin (DA); desamino-diketo metribuzin (DADK)
Abstract Metribuzin (MB) is used for control of weeds in crops like potato, maize and sugarcane. Its extensive and unjudicial use has resulted in various environmental issues; hence it is very critical to remediate this herbicide at the respective point source. Plant associated, MB degrading bacterial strains, Rhodococcus rhodochrous sp. AQ1, Bacillus tequilensis sp. AQ2, Bacillus aryabhattai sp. AQ3 and Bacillus safensis sp. AQ4 were isolated, and a consortium MB3R was developed. For degradation of MB by the consortium MB3R, various parameters i.e., pH, temperature, inoculum density and pesticide concentration were optimized by using Taguchi design of experiment (DOE). MB degradation was dependent upon all the four factors. The contribution of each factor on MB degradation was according to the order: temperature > inoculum density > pH > pesticide concentration. Fitness of Taguchi DOE in forecasting the optimum response, was confirmed experimentally by using optimized levels of the four factors i.e., pH 7.0, temperature 30 °C, pesticide concentration 45 mg l-1 and an inoculum density of 5.0 × 10 5 CFU ml-1 whereby 98.63 % MB degradation was observed. Appearance and subsequent degradation of three MB metabolites, desamino-metribuzin (DA), diketo-metribuzin (DK) and desamino-diketo-metribuzin (DADK) during biodegradation by the consortium was observed.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=21116
Wahla, A.Q., Iqbal, S., Anwar, S., Firdous, S., Müller, J.A. (2019):
Optimizing the metribuzin degrading potential of a novel bacterial consortium based on Taguchi design of experiment
J. Hazard. Mater. 366 , 1 - 9 10.1016/j.jhazmat.2018.11.054