Publication Details |
Category | Text Publication |
Reference Category | Journals |
DOI | 10.1016/j.jes.2018.09.008 |
Title (Primary) | Individual stages of bacterial dichloromethane degradation mapped by stable carbon and chlorine isotope analysis |
Author | Torgonskaya, M.L.; Zyakun, A.M.; Trotsenko, Y.A.; Laurinavichius, K.S.; Kümmel, S.
![]() |
Source Titel | Journal of Environmental Sciences |
Year | 2019 |
Department | ISOBIO |
Volume | 78 |
Page From | 147 |
Page To | 160 |
Language | englisch |
Keywords | Dichloromethane (DCM); Microbial degradation; Aerobic methylotrophic bacteria; Dichloromethane dehalogenase; Fractionation of stable carbon and chlorine isotopes |
UFZ wide themes | ProVIS; |
Abstract | The fractionation of stable carbon and chlorine isotopes of dichloromethane (CH2Cl2, DCM) upon dechlorination by cells of the aerobic methylotroph Methylobacterium extorquens DM4 and by purified DCM dehalogenases of the glutathione S-transferase family was analyzed. Isotope effects for individual steps of the multi-stage DCM degradation process, including transfer across the cell wall from the aqueous medium to the cell cytoplasm, dehalogenase binding, and catalytic reaction, were considered. The observed carbon and chlorine isotope fractionation accompanying DCM consumption by cell supensions and enzymes was mainly determined by the breaking of C |
Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=21026 |
Torgonskaya, M.L., Zyakun, A.M., Trotsenko, Y.A., Laurinavichius, K.S., Kümmel, S., Vuilleumier, S., Richnow, H.H. (2019): Individual stages of bacterial dichloromethane degradation mapped by stable carbon and chlorine isotope analysis J. Environ. Sci. 78 , 147 - 160 10.1016/j.jes.2018.09.008 |